Literature DB >> 896478

Poly-2'-deoxy-2'-fluoro-cytidylic acid: enzymatic synthesis, spectroscopic characterization and interaction with poly-inosinic acid.

W Guschlbauer, M Blandin, J L Drocourt, M N Thang.   

Abstract

The polymerization of 2'deoxy-2'-fluoro-cytidine-diphosphate (dCflDP) by polynucleotide phosphorylase is barely detectable in the presence of Mg++ under usual experimental conditions for polymerization of nucleoside diphosphates. High concentrations of enzyme have to be used to accomplish the synthesis. Mn++ is a better activator than Mg++ for the reaction. cCflDP inhibits the polymerization of CDP and has a Km=8.8X10-3M, six times higher than CDP.- The polymer, poly (dCfl), ressembles in many respects poly(C), but not poly(dC): the acid selfstructure forms at similar pK's; interaction with poly(I) yields a 1:1 complex the CD spectrum of which is similar to that of poly(I).poly(C). Finally, the Tm's of poly(I).poly(dCfl) are comparable to those of poly(I).poly(C).

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Year:  1977        PMID： 896478      PMCID： PMC342532          DOI： 10.1093/nar/4.6.1933

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  28 in total

1.  HELIX--RANDOM COIL TRANSITIONS IN DNA HOMOPOLYMER PAIRS.

Authors:  R B INMAN; R L BALDWIN
Journal:  J Mol Biol       Date:  1964-04       Impact factor: 5.469

2.  PHYSICAL AND CHEMICAL CHARACTERIZATION OF THE ORDERED COMPLEXES FORMED BETWEEN POLYINOSINIC ACID, POLYCYTIDYLIC ACID AND THEIR DEOXYRIBO-ANALOGUES.

Authors:  M J CHAMBERLIN; D L PATTERSON
Journal:  J Mol Biol       Date:  1965-06       Impact factor: 5.469

3.  Single-stranded oligomers and polymers of cytidylic and 2'-deoxycytidylic acids: comparative optical rotatory studies.

Authors:  B A Adler; L Grossman; G D Fasman
Journal:  Proc Natl Acad Sci U S A       Date:  1967-02       Impact factor: 11.205

4.  Physical and enzymatic studies on poly d(I-C)-poly d(I-C), an unusual double-helical DNA.

Authors:  Y Mitsui; R Langridge; B E Shortle; C R Cantor; R C Grant; M Kodama; R D Wells
Journal:  Nature       Date:  1970-12-19       Impact factor: 49.962

5.  Nucleoside conformations. 16. Nuclear magnetic resonance and circular dichroism studies on pyrimidine-2'-fluoro-2'-deoxyribonucleosides.

Authors:  M Blandin; J C Catlin; W Guschlbauer
Journal:  Biochim Biophys Acta       Date:  1974-09-13

6.  [Purification and characterization of the polynucleotide phosphorylase of Azotobacter vinelandii].

Authors:  D C Thang
Journal:  Bull Soc Chim Biol (Paris)       Date:  1967

7.  Molecular and crystal structure of the polynucleotide complex: polyinosinic acid plus polydeoxycytidylic acid.

Authors:  E J O'Brien; A W MacEwan
Journal:  J Mol Biol       Date:  1970-03-14       Impact factor: 5.469

8.  Protonated polynucleotide structures. IX. Disproportionation of poly (G)-poly (C) in acid medium.

Authors:  D Thiele; W Guschlbauer
Journal:  Biopolymers       Date:  1971       Impact factor: 2.505

9.  A novel method for phosphorylation of nucleosides to 5'-nucleotides.

Authors:  M Yoshikawa; T Kato; T Takenishi
Journal:  Tetrahedron Lett       Date:  1967-12       Impact factor: 2.415

10.  CONVERSION OF MONO- AND OLIGODEOXYRIBONUCLEOTIDES TO 5-TRIPHOSPHATES.

Authors:  D E HOARD; D G OTT
Journal:  J Am Chem Soc       Date:  1965-04-20       Impact factor: 15.419
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  8 in total

1.  Molecular requirements for degradation of a modified sense RNA strand by Escherichia coli ribonuclease H1.

Authors:  Daniel R Yazbeck; Kyung-Lyum Min; Masad J Damha
Journal:  Nucleic Acids Res       Date:  2002-07-15       Impact factor: 16.971

2.  2'-Deoxy-2'-fluorouridine-5'-triphosphates: a possible substrate for E. coli RNA polymerase.

Authors:  D Pinto; M T Sarocchi-Landousy; W Guschlbauer
Journal:  Nucleic Acids Res       Date:  1979-03       Impact factor: 16.971

3.  2'-Fluoro modified nucleic acids: polymerase-directed synthesis, properties and stability to analysis by matrix-assisted laser desorption/ionization mass spectrometry.

Authors:  T Ono; M Scalf; L M Smith
Journal:  Nucleic Acids Res       Date:  1997-11-15       Impact factor: 16.971

4.  2'-Deoxy-2'-fluorouridine-5'-phosphate: an alternative substrate for thymidylate synthetase from Escherichia coli K12.

Authors:  F Wohlrab; T Haertlé; T Trichtinger; W Guschlbauer
Journal:  Nucleic Acids Res       Date:  1978-12       Impact factor: 16.971

5.  Specific interaction of netropsin, distamycin-3 and analogs with LC duplexes: reversion towards the B form of the 2'-deoxy-.2'-deoxy-2'-fluoro-hybrid duplexes upon specific interaction with netropsin, distamycin-3 and analogs.

Authors:  C Marck; N Kakiuchi; W Guschlbauer
Journal:  Nucleic Acids Res       Date:  1982-10-11       Impact factor: 16.971

6.  Differential stabilization by netropsin of inducible B-like conformations in deoxyribo-, ribo- and 2'-deoxy-2'-fluororibo-adenosine containing duplexes of (dA)n . (dT)n and (dA)n . (dU)na.

Authors:  C Zimmer; N Kakiuchi; W Guschlbauer
Journal:  Nucleic Acids Res       Date:  1982-03-11       Impact factor: 16.971

7.  Nucleoside conformation is determined by the electronegativity of the sugar substituent.

Authors:  W Guschlbauer; K Jankowski
Journal:  Nucleic Acids Res       Date:  1980-03-25       Impact factor: 16.971

8.  Polynucleotides. LVI. Synthesis and properties of poly(2-deoxy-2'-fluoroinosinic acid).

Authors:  M Ikehara; N Kakiuchi; T Fukui
Journal:  Nucleic Acids Res       Date:  1978-09       Impact factor: 16.971
  8 in total

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