Literature DB >> 370779

2'-Deoxy-2'-fluorouridine-5'-phosphate: an alternative substrate for thymidylate synthetase from Escherichia coli K12.

F Wohlrab, T Haertlé, T Trichtinger, W Guschlbauer.   

Abstract

The substrate specificity of 2'-deoxy-2'-substituted uridines and their 5'-phosphates towards thymidylate synthetase from Escherichia coli K12 was investigated. Besides the natural substrate 2'-deoxyuridine-5'-phosphate (dUMP), only 2'-deoxy-2'-fluorouridine-5'-phosphate (dUflMP) was a substrate. The KM of dUflMP is 11 times higher than that of dUMP, while the Vmax values are virtually the same. It is concluded that the size of the 2'-substituent and not its polarity (and the concomitant conformational change) determines substrate specificity of thymidylate synthetase.

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Year:  1978        PMID： 370779      PMCID： PMC342786          DOI： 10.1093/nar/5.12.4753

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  28 in total

1.  FLUORINATED PYRIMIDINES. XXII. EFFECTS OF VARIOUS COMPOUNDS ON THE INCORPORATION OF (14C)FORMATE INTO DNA THYMINE IN SUSPENSIONS OF EHRLICH ASCITES CELLS.

Authors:  C HEIDELBERGER; J BOOHAR; G D BIRNIE
Journal:  Biochim Biophys Acta       Date:  1964-12-16

2.  FLUORINATED PYRIMIDINES. XXV. THE INHIBITION OF THYMIDYLATE SYNTHETASE FROM EHRLICH ASCITES CARCINOMA CELLS BY PYRIMIDINE ANALOGS.

Authors:  P REYES; C HEIDELBERGER
Journal:  Biochim Biophys Acta       Date:  1965-05-11

3.  The enzymatic synthesis of thymidylate. I. Early steps in the purification of thymidylate synthetase of Escherichia coli.

Authors:  A J WAHBA; M FRIEDKIN
Journal:  J Biol Chem       Date:  1962-12       Impact factor: 5.157

Review 4.  Thymidylate synthetase - a target enzyme in cancer chemotherapy.

Authors:  P V Danenberg
Journal:  Biochim Biophys Acta       Date:  1977-12-23

5.  Poly-2'-deoxy-2'-fluoro-cytidylic acid: enzymatic synthesis, spectroscopic characterization and interaction with poly-inosinic acid.

Authors:  W Guschlbauer; M Blandin; J L Drocourt; M N Thang
Journal:  Nucleic Acids Res       Date:  1977-06       Impact factor: 16.971

6.  19F nuclear magnetic resonance investigation of the ternary complex formed between native thymidylate synthetase, 5-fluoro-2'-deoxyuridylate, and 5,10-methylenetetrahydrofolate.

Authors:  R A Byrd; W H Dawson; P D Ellis; R B Dunlap
Journal:  J Am Chem Soc       Date:  1977-08-31       Impact factor: 15.419

7.  A kinetic study of thymidylate synthase from Lactobacillus casei.

Authors:  H H Daron; J L Aull
Journal:  J Biol Chem       Date:  1978-02-10       Impact factor: 5.157

8.  [Thymidylate synthetase (author's transl)].

Authors:  W Rode
Journal:  Postepy Biochem       Date:  1977

9.  Thymidylate synthetase: fluorine-19 nmr characterization of the active site peptide covalently bound to 5-fluoro-2'-deoxyuridylate and 5,10-methylenetetrahydrofolate.

Authors:  T L James; A L Pogolotti; K M Ivanetich; Y Wataya; S S Lam; D V Santi
Journal:  Biochem Biophys Res Commun       Date:  1976-09-20       Impact factor: 3.575

10.  Polynucleotides. LII. Synthesis and properties of poly(2'-deoxy-2'-fluoroadenylic acid).

Authors:  M Ikehara; T Fukui; N Kakiuchi
Journal:  Nucleic Acids Res       Date:  1978-06       Impact factor: 16.971
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  3 in total

1.  2'-Deoxy-2'-fluorouridine-5'-triphosphates: a possible substrate for E. coli RNA polymerase.

Authors:  D Pinto; M T Sarocchi-Landousy; W Guschlbauer
Journal:  Nucleic Acids Res       Date:  1979-03       Impact factor: 16.971

2.  Specific interaction of netropsin, distamycin-3 and analogs with LC duplexes: reversion towards the B form of the 2'-deoxy-.2'-deoxy-2'-fluoro-hybrid duplexes upon specific interaction with netropsin, distamycin-3 and analogs.

Authors:  C Marck; N Kakiuchi; W Guschlbauer
Journal:  Nucleic Acids Res       Date:  1982-10-11       Impact factor: 16.971

3.  CoFactor: Folate Requirement for Optimization of 5-Fluouracil Activity in Anticancer Chemotherapy.

Authors:  Muhammad Wasif Saif; Nektaria Makrilia; Kostas Syrigos
Journal:  J Oncol       Date:  2010-12-16       Impact factor: 4.375
  3 in total

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