| Literature DB >> 8955417 |
Abstract
The bluF gene of Rhodobacter capsulatus is the first gene of the bluFEDCB operon which is involved in late steps of the cobalamin synthesis. To determine the function of the bluF gene product, a bluF::omega-Km mutant strain was constructed and characterized. This vitamin B12 auxotrophic mutant strain shows a 3.5-times higher vitamin B12 requirement under phototrophic growth conditions than under chemotrophic growth conditions. Surprisingly, the bluF promoter activity does not respond to alterations to the oxygen tension or vitamin B12 concentration.Entities:
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Year: 1996 PMID: 8955417 PMCID: PMC178648 DOI: 10.1128/jb.178.24.7308-7310.1996
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490