Literature DB >> 8955363

The recombinant catalytic domain of membrane-type matrix metalloproteinase-1 (MT1-MMP) induces activation of progelatinase A and progelatinase A complexed with TIMP-2.

A Lichte1, H Kolkenbrock, H Tschesche.   

Abstract

A truncated form of the membrane-type matrix metalloproteinase-1 [(Ala21-Ile318)proMT1-MMP] lacking the hemopexin-like and trans-membrane domain was produced in E. coli. We demonstrate that the recombinant proenzyme was autoproteolytically processed to a fully active catalytic domain with N-terminal Ile114. The catalytic domain of MT1-MMP initiated the activation of progelatinase A and progelatinase A complexed with tissue inhibitor of metalloproteinases-2 (TIMP-2). As a typical soluble metalloproteinase it was able to cleave physiologic as well as synthetic substrates. Our kinetic data demonstrate that TIMP-2 is a potent inhibitor for the recombinant enzyme.

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Year:  1996        PMID： 8955363     DOI： 10.1016/s0014-5793(96)01206-9

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  14 in total

1.  Analysis of tissue inhibitor of metalloproteinases-2 effect on pro-matrix metalloproteinase-2 activation by membrane-type 1 matrix metalloproteinase using baculovirus/insect-cell expression system.

Authors:  Y Jo; J Yeon; H J Kim; S T Lee
Journal:  Biochem J       Date:  2000-02-01       Impact factor: 3.857

2.  Functional Production of Catalytic Domains of Human MMPs in Escherichia coli Periplasm.

Authors:  Dong Hyun Nam; Ki Baek Lee; Xin Ge
Journal:  Methods Mol Biol       Date:  2018

3.  Inhibition of matrix metalloproteinase 14 (MMP-14)-mediated cancer cell migration.

Authors:  Kevin Zarrabi; Antoine Dufour; Jian Li; Cem Kuscu; Ashleigh Pulkoski-Gross; Jizu Zhi; Youjun Hu; Nicole S Sampson; Stanley Zucker; Jian Cao
Journal:  J Biol Chem       Date:  2011-07-27       Impact factor: 5.157

4.  Solitary lung tumors and their spontaneous metastasis in athymic nude mice orthotopically implanted with human non-small cell lung cancer.

Authors:  T Yamaura; K Murakami; Y Doki; S Sugiyama; T Misaki; Y Yamada; I Saiki
Journal:  Neoplasia       Date:  2000 Jul-Aug       Impact factor: 5.715

5.  Internal cleavages of the autoinhibitory prodomain are required for membrane type 1 matrix metalloproteinase activation, although furin cleavage alone generates inactive proteinase.

Authors:  Vladislav S Golubkov; Piotr Cieplak; Alexei V Chekanov; Boris I Ratnikov; Alexander E Aleshin; Natalya V Golubkova; Tatiana I Postnova; Ilian A Radichev; Dmitri V Rozanov; Wenhong Zhu; Khatereh Motamedchaboki; Alex Y Strongin
Journal:  J Biol Chem       Date:  2010-07-06       Impact factor: 5.157

6.  Biochemical characterization of the cellular glycosylphosphatidylinositol-linked membrane type-6 matrix metalloproteinase.

Authors:  Ilian A Radichev; Albert G Remacle; Sergey A Shiryaev; Angela N Purves; Sherida L Johnson; Maurizio Pellecchia; Alex Y Strongin
Journal:  J Biol Chem       Date:  2010-03-22       Impact factor: 5.157

7.  Inhibition of enzyme activity of and cell-mediated substrate cleavage by membrane type 1 matrix metalloproteinase by newly developed mercaptosulphide inhibitors.

Authors:  Douglas R Hurst; Martin A Schwartz; Yonghao Jin; Mohammad A Ghaffari; Pallavi Kozarekar; Jian Cao; Qing-Xiang Amy Sang
Journal:  Biochem J       Date:  2005-12-15       Impact factor: 3.857

8.  Crystal structure of the complex formed by the membrane type 1-matrix metalloproteinase with the tissue inhibitor of metalloproteinases-2, the soluble progelatinase A receptor.

Authors:  C Fernandez-Catalan; W Bode; R Huber; D Turk; J J Calvete; A Lichte; H Tschesche; K Maskos
Journal:  EMBO J       Date:  1998-09-01       Impact factor: 11.598

9.  Catalytic- and ecto-domains of membrane type 1-matrix metalloproteinase have similar inhibition profiles but distinct endopeptidase activities.

Authors:  Douglas R Hurst; Martin A Schwartz; Mohammad A Ghaffari; Yonghao Jin; Harald Tschesche; Gregg B Fields; Qing-Xiang Amy Sang
Journal:  Biochem J       Date:  2004-02-01       Impact factor: 3.857

10.  Membrane type 1 matrix metalloproteinase (MT1-MMP) cleaves the recombinant aggrecan substrate rAgg1mut at the 'aggrecanase' and the MMP sites. Characterization of MT1-MMP catabolic activities on the interglobular domain of aggrecan.

Authors:  F H Büttner; C E Hughes; D Margerie; A Lichte; H Tschesche; B Caterson; E Bartnik
Journal:  Biochem J       Date:  1998-07-01       Impact factor: 3.857
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