| Literature DB >> 29318544 |
Dong Hyun Nam1, Ki Baek Lee1, Xin Ge2.
Abstract
Due to their central roles in tumor growth and invasion, milligram-level amounts of active MMPs are frequently required for cancer research and development of chemical or biological MMP inhibitors. Here we describe methods for functional production of catalytic domains of MMPs (cdMMPs) in E. coli periplasm without refolding or activation process. We demonstrate applications of this straightforward approach for cdMMP-9, cdMMP-14, and cdMMP-14 mutants.Entities:
Keywords: Catalytic domain; MMP; Periplasmic expression
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Year: 2018 PMID： 29318544 PMCID： PMC5901902 DOI： 10.1007/978-1-4939-7595-2_7
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745