Literature DB >> 895217

Purification of bovine milk lipoprotein lipase with the aid of detergent.

P K Kinnunen.   

Abstract

The present study describes a simple method for the purification of bovine milk lipoprotein lipase based on affinity chromatography on agarose containing covalently linked heparin and the use of a non-ionic detergent, Triton X-100. By this procedure miligram amounts of detergent-free lipoprotein-ionic lipase with a specific activity of 28.9 mmoles free fatty acid/mg protein/mg protein/hour can be obtained. The apparent molecular weight of the polypeptide as determined by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate is 55,000. The purified triacylglycerol lipase also hydrolyzes monoacylglycerol, but the activity against this lipid is 40 times lower than that against triacylglycerol.

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Year:  1977        PMID： 895217

Source DB:  PubMed          Journal:  Med Biol        ISSN： 0302-2137


  7 in total

1.  Lipoprotein Lipase Interaction with Synthetic N-Dansyl Fragments of Apolipoprotein C-II.

Authors:  L C Smith; J C Voyta; P K Kinnunen; A M Gotto; J T Sparrow
Journal:  Biophys J       Date:  1982-01       Impact factor: 4.033

2.  VLDL substrate properties and efficiency of their metabolic transformation by LPL.

Authors:  A D Dergunov; V V Shuvaev; N V Perova
Journal:  Experientia       Date:  1989-05-15

3.  Purification and characterization of rat adipose tissue lipoprotein lipase.

Authors:  S M Parkin; B K Speake; D S Robinson
Journal:  Biochem J       Date:  1982-12-01       Impact factor: 3.857

4.  Activation of lipoprotein lipase by native and synthetic fragments of human plasma apolipoprotein C-II.

Authors:  P K Kinnunen; R L Jackson; L C Smith; A M Gotto; J T Sparrow
Journal:  Proc Natl Acad Sci U S A       Date:  1977-11       Impact factor: 11.205

5.  Role of apolipoprotein E in the lipolytic conversion of beta-very low density lipoproteins to low density lipoproteins in type III hyperlipoproteinemia.

Authors:  C Ehnholm; R W Mahley; D A Chappell; K H Weisgraber; E Ludwig; J L Witztum
Journal:  Proc Natl Acad Sci U S A       Date:  1984-09       Impact factor: 11.205

6.  The hydrolysis of very low density lipoproteins and chylomicrons of intestinal origin by lipoprotein lipase in ruminants.

Authors:  W W Christie; R C Noble; R A Clegg
Journal:  Lipids       Date:  1986-03       Impact factor: 1.880

7.  Recombinant gamma interferon induces hypertriglyceridemia and inhibits post-heparin lipase activity in cancer patients.

Authors:  R Kurzrock; M F Rohde; J R Quesada; S H Gianturco; W A Bradley; S A Sherwin; J U Gutterman
Journal:  J Exp Med       Date:  1986-10-01       Impact factor: 14.307
  7 in total

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