Literature DB >> 8945596

Cloning and characterization of the gene encoding the OmpU outer membrane protein of Vibrio cholerae.

V Sperandio1, C Bailey, J A Girón, V J DiRita, W D Silveira, A L Vettore, J B Kaper.   

Abstract

The OmpU outer membrane protein is a member of the ToxR regulon of Vibrio cholerae and has recently been shown to be a potential adherence factor for this species. Using PCR and degenerate oligonucleotide primers based on internal peptide sequences of purified OmpU, we have cloned and sequenced the gene encoding OmpU. The ompU gene is predicted to encode a 36,646-molecular-weight protein which is present in both cholera toxin-positive and -negative V. cholerae O1 and O139 strains.

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Year:  1996        PMID： 8945596      PMCID： PMC174538          DOI： 10.1128/iai.64.12.5406-5409.1996

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  20 in total

1.  Genetic footprint on the ToxR-binding site in the promoter for cholera toxin.

Authors:  J D Pfau; R K Taylor
Journal:  Mol Microbiol       Date:  1996-04       Impact factor: 3.501

2.  A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR.

Authors:  V L Miller; J J Mekalanos
Journal:  J Bacteriol       Date:  1988-06       Impact factor: 3.490

3.  A small cosmid for efficient cloning of large DNA fragments.

Authors:  B Hohn; J Collins
Journal:  Gene       Date:  1980-11       Impact factor: 3.688

4.  A comprehensive set of sequence analysis programs for the VAX.

Authors:  J Devereux; P Haeberli; O Smithies
Journal:  Nucleic Acids Res       Date:  1984-01-11       Impact factor: 16.971

5.  The pathogenicity of nonenterotoxigenic Vibrio cholerae serogroup O1 biotype El Tor isolated from sewage water in Brazil.

Authors:  M M Levine; R E Black; M L Clements; L Cisneros; A Saah; D R Nalin; D M Gill; J P Craig; C R Young; P Ristaino
Journal:  J Infect Dis       Date:  1982-03       Impact factor: 5.226

6.  The OmpU outer membrane protein, a potential adherence factor of Vibrio cholerae.

Authors:  V Sperandio; J A Girón; W D Silveira; J B Kaper
Journal:  Infect Immun       Date:  1995-11       Impact factor: 3.441

7.  Synthesis of cholera toxin is positively regulated at the transcriptional level by toxR.

Authors:  V L Miller; J J Mekalanos
Journal:  Proc Natl Acad Sci U S A       Date:  1984-06       Impact factor: 11.205

8.  Use of phoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin.

Authors:  R K Taylor; V L Miller; D B Furlong; J J Mekalanos
Journal:  Proc Natl Acad Sci U S A       Date:  1987-05       Impact factor: 11.205

9.  Porins of Vibrio cholerae: purification and characterization of OmpU.

Authors:  S R Chakrabarti; K Chaudhuri; K Sen; J Das
Journal:  J Bacteriol       Date:  1996-01       Impact factor: 3.490

10.  Molecular cloning and characterization of the structural gene for protein I, the major outer membrane protein of Neisseria gonorrhoeae.

Authors:  N H Carbonetti; P F Sparling
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205
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  20 in total

1.  Mutations in toxR and toxS that separate transcriptional activation from DNA binding at the cholera toxin gene promoter.

Authors:  J D Pfau; R K Taylor
Journal:  J Bacteriol       Date:  1998-09       Impact factor: 3.490

2.  The toxR gene of Vibrio (Listonella) anguillarum controls expression of the major outer membrane proteins but not virulence in a natural host model.

Authors:  J Okuda; T Nakai; P S Chang; T Oh; T Nishino; T Koitabashi; M Nishibuchi
Journal:  Infect Immun       Date:  2001-10       Impact factor: 3.441

3.  Vibrio cholerae OmpU and OmpT porins are differentially affected by bile.

Authors:  Jamie A Wibbenmeyer; Daniele Provenzano; Candice F Landry; Karl E Klose; Anne H Delcour
Journal:  Infect Immun       Date:  2002-01       Impact factor: 3.441

4.  Genotypes associated with virulence in environmental isolates of Vibrio cholerae.

Authors:  I N Rivera; J Chun; A Huq; R B Sack; R R Colwell
Journal:  Appl Environ Microbiol       Date:  2001-06       Impact factor: 4.792

5.  Characterization of the role of the ToxR-modulated outer membrane porins OmpU and OmpT in Vibrio cholerae virulence.

Authors:  D Provenzano; C M Lauriano; K E Klose
Journal:  J Bacteriol       Date:  2001-06       Impact factor: 3.490

6.  The ToxR-mediated organic acid tolerance response of Vibrio cholerae requires OmpU.

Authors:  D S Merrell; C Bailey; J B Kaper; A Camilli
Journal:  J Bacteriol       Date:  2001-05       Impact factor: 3.490

7.  Protection against development of otitis media induced by nontypeable Haemophilus influenzae by both active and passive immunization in a chinchilla model of virus-bacterium superinfection.

Authors:  L O Bakaletz; B J Kennedy; L A Novotny; G Duquesne; J Cohen; Y Lobet
Journal:  Infect Immun       Date:  1999-06       Impact factor: 3.441

8.  RNA arbitrarily primed PCR survey of genes regulated by ToxR in the deep-sea bacterium Photobacterium profundum strain SS9.

Authors:  K A Bidle; D H Bartlett
Journal:  J Bacteriol       Date:  2001-03       Impact factor: 3.490

9.  Virulence regulator AphB enhances toxR transcription in Vibrio cholerae.

Authors:  Xiao Xu; Andrew M Stern; Zhi Liu; Biao Kan; Jun Zhu
Journal:  BMC Microbiol       Date:  2010-01-06       Impact factor: 3.605

10.  High-throughput sequencing reveals suppressors of Vibrio cholerae rpoE mutations: one fewer porin is enough.

Authors:  Brigid M Davis; Matthew K Waldor
Journal:  Nucleic Acids Res       Date:  2009-07-20       Impact factor: 16.971
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