Literature DB >> 8940420

Cloning and sequence of a region of Vibrio cholerae O139 Bengal and its use in PCR-based detection.

S Falklind1, M Stark, M J Albert, M Uhlén, J Lundeberg, A Weintraub.   

Abstract

We isolated and characterized a Vibrio cholerae O139 Bengal-specific DNA region by arbitrary PCR. The fragment contains open reading frames encoding two potential glycosyltransferases possibly involved in capsular polysaccharide or lipopolysaccharide biosynthesis. In order to evaluate the possibility that this region could be used for the specific detection of V. cholerae O139 Bengal, a PCR system was established. The specificity and sensitivity of the PCR were investigated by analyzing 240 strains within the family Vibrionaceae and 178 stains of other gram-negative bacteria. All V. cholerae O139 Bengal strains tested were positive, and none of the 384 control strains were amplified. The sensitivity of the assay was 10(2) CFU/ml.

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Year:  1996        PMID： 8940420      PMCID： PMC229431          DOI： 10.1128/jcm.34.12.2904-2908.1996

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  31 in total

1.  Bidirectional solid-phase sequencing of in vitro-amplified plasmid DNA.

Authors:  T Hultman; S Bergh; T Moks; M Uhlén
Journal:  Biotechniques       Date:  1991-01       Impact factor: 1.993

2.  Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support.

Authors:  T Hultman; S Ståhl; E Hornes; M Uhlén
Journal:  Nucleic Acids Res       Date:  1989-07-11       Impact factor: 16.971

3.  Large outbreak of clinical cholera due to Vibrio cholerae non-O1 in Bangladesh.

Authors:  M J Albert; A K Siddique; M S Islam; A S Faruque; M Ansaruzzaman; S M Faruque; R B Sack
Journal:  Lancet       Date:  1993-03-13       Impact factor: 79.321

4.  Outbreak of Vibrio cholerae non-O1 in India and Bangladesh.

Authors:  M K Bhattacharya; S K Bhattacharya; S Garg; P K Saha; D Dutta; G B Nair; B C Deb; K P Das
Journal:  Lancet       Date:  1993-05-22       Impact factor: 79.321

5.  pUR 250 allows rapid chemical sequencing of both DNA strands of its inserts.

Authors:  U Rüther
Journal:  Nucleic Acids Res       Date:  1982-10-11       Impact factor: 16.971

6.  [The structure of the O-specific polysaccharide chain of the lipopolysaccharide of Yersinia pseudotuberculosis serovar VII].

Authors:  N A Kotandrova; R P Gorshkova; V A Zubkov; Iu S Ovodov
Journal:  Bioorg Khim       Date:  1989-01

7.  Detection of Shigella dysenteriae type 1 and Shigella flexneri in feces by immunomagnetic isolation and polymerase chain reaction.

Authors:  D Islam; A A Lindberg
Journal:  J Clin Microbiol       Date:  1992-11       Impact factor: 5.948

8.  Structural studies of the O-antigens from Salmonella greenside and Salmonella adelaide.

Authors:  L Kenne; B Lindberg; E Söderholm; D R Bundle; D W Griffith
Journal:  Carbohydr Res       Date:  1983-01-01       Impact factor: 2.104

9.  Identification and sequence of the gene for abequose synthase, which confers antigenic specificity on group B salmonellae: homology with galactose epimerase.

Authors:  P Wyk; P Reeves
Journal:  J Bacteriol       Date:  1989-10       Impact factor: 3.490

10.  Identification and sequence of rfbS and rfbE, which determine antigenic specificity of group A and group D salmonellae.

Authors:  N Verma; P Reeves
Journal:  J Bacteriol       Date:  1989-10       Impact factor: 3.490
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  2 in total

1.  PCR-based detection of Vibrio cholerae O139 Bengal with sequences encoding glycosyltransferases.

Authors:  S C Arya
Journal:  J Clin Microbiol       Date:  1997-12       Impact factor: 5.948

2.  Rapid detection of Vibrio cholerae O139 Bengal from stool specimens by PCR.

Authors:  M J Albert; D Islam; S Nahar; F Qadri; S Falklind; A Weintraub
Journal:  J Clin Microbiol       Date:  1997-06       Impact factor: 5.948
  2 in total

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