| Literature DB >> 8921848 |
G W Sundin1, S P Kidambi, M Ullrich, C L Bender.
Abstract
The indigenous plasmids, pPSR1 and pPSR5, were each shown to confer resistance to ultraviolet light (UV) in Pseudomonas syringae (Ps) pv. syringae FF5. The UV-resistance (UVR) determinant was subcloned from a cosmid library of pPSR1, and sequence analysis revealed the presence of two ORFs, designated rulAB which are homologous to the Escherichia coli umuDC mutagenic DNA repair systems and other plasmid-encoded UVR operons. Amino acid (aa) alignments indicated that RulAB are most closely related to the RumAB proteins from plasmid R391, sharing 40.5% and 48.6% aa identity with RumA and RumB, respectively. UV sensitivity assays with the cloned rulAB genes indicated that the expression of UVR in Ps required a functional recA gene.Entities:
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Year: 1996 PMID： 8921848 DOI： 10.1016/0378-1119(96)00273-9
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688