Literature DB >> 8905621

Leishmania donovani: metabolite mapping of promastigotes using proton nuclear magnetic resonance spectroscopy.

U K Singha1, V Bhakuni, V Ali, R Roy.   

Abstract

Proton nuclear magnetic resonance spectroscopy was used for studying the intracellular metabolite profile of promastigotes of Leishmania donovani. The major intracellular metabolites observed in the promastigotes were acetate, alanine, succinate, glycine, alpha-glycerophosphorylcholine, acetoacetate, arginine and ethanol. A comparative study of the intracellular metabolite profile of promastigotes of different strains of L. donovani showed that, all the major intracellular metabolites were present in promastigotes of different strains. A quantitative estimation of metabolites showed a strain specific (Finger print) metabolite profile which can be used for strain/species identification/differentiation.

Entities:  

Mesh：

Substances：

Year:  1996        PMID： 8905621     DOI： 10.1007/bf00250991

Source DB:  PubMed          Journal:  Mol Cell Biochem        ISSN： 0300-8177            Impact factor:   3.396


  11 in total

1.  The metabolism of Leishmania donovani in culture.

Authors:  S CROWTHER; J D FULTON; L P JOYNER
Journal:  Biochem J       Date:  1954-02       Impact factor: 3.857

2.  Metabolite mapping of Toxocara canis using one- and two-dimensional proton magnetic resonance spectroscopy.

Authors:  M P Learmonth; M R Euerby; D E Jacobs; W A Gibbons
Journal:  Mol Biochem Parasitol       Date:  1987-10       Impact factor: 1.759

Review 3.  Biochemistry of the Leishmania species.

Authors:  R H Glew; A K Saha; S Das; A T Remaley
Journal:  Microbiol Rev       Date:  1988-12

4.  Proton nuclear magnetic resonance spectroscopy of aqueous solutions: complete elimination of the water resonance by spin-spin relaxation.

Authors:  D L Rabenstein; S Y Fan
Journal:  Anal Chem       Date:  1986-12       Impact factor: 6.986

5.  Anaerobic formation of succinate from glucose and bicarbonate in resting cells of Leishmania donovani.

Authors:  T Chatterjee; A G Datta
Journal:  Exp Parasitol       Date:  1973-02       Impact factor: 2.011

6.  A comparative study of D-lactate, L-lactate and glycerol formation by four species of Leishmania and by Trypanosoma lewisi and Trypanosoma brucei gambiense.

Authors:  T N Darling; A E Balber; J J Blum
Journal:  Mol Biochem Parasitol       Date:  1988-09       Impact factor: 1.759

7.  Metabolic studies of the protozoan parasite, Crithidia luciliae, using proton nuclear magnetic resonance spectroscopy.

Authors:  F V Gilroy; M R Edwards; R S Norton; W J O'Sullivan
Journal:  Mol Biochem Parasitol       Date:  1988-11       Impact factor: 1.759

8.  A carbon-13 nuclear magnetic resonance analysis of the products of glucose metabolism in Leishmania pifanoi amastigotes and promastigotes.

Authors:  P M Rainey; N E MacKenzie
Journal:  Mol Biochem Parasitol       Date:  1991-04       Impact factor: 1.759

9.  The free amino acid pool in Leishmania tropica promastigotes.

Authors:  M W Simon; K Jayasimhulu; A J Mukkada
Journal:  Mol Biochem Parasitol       Date:  1983-09       Impact factor: 1.759

10.  Proton NMR lipid profile of Leishmania donovani promastigotes.

Authors:  R K Adosraku; M M Anderson; G J Anderson; G Choi; S L Croft; V Yardley; J D Phillipson; W A Gibbons
Journal:  Mol Biochem Parasitol       Date:  1993-12       Impact factor: 1.759
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.