The free amino acid pool in Leishmania tropica promastigotes.

The principal constituents of the free amino acid pool in Leishmania tropica promastigotes include alanine, glycine, serine, threonine, alpha-aminobutyric acid and ethanolamine. Changes in levels of the pool constituents during starvation, nutrient supplementation and treatment with a metabolic inhibitor (sodium fluoride) were followed. Several enzymes capable of converting amino acids to Krebs cycle intermediates were identified. It is suggested that during prolonged starvation which is accompanied by enhanced proteolysis, these enzymes enable the cells to utilize endogenous amino acids as carbon and energy sources. This is compatible with previous reports that Leishmania promastigotes in culture could grow on amino acids as growth substrates when carbohydrates are totally lacking.