PURPOSE: We report our experience of freezing human embryos using an ultrarapid freezing method. METHODS: The patients were superovulated. Oocytes were inseminated and cultured in HTF + 10% serum. A maximum of three embryos was transferred and the rest of the embryos were frozen ultrarapidly after a 3-min equilibration period in PB1 + 3.5 M DMSO + 0.25 M sucrose. Embryos were thawed in a 37 degrees C water bath for 6 sec, then cultured in PB1 + 20% serum for 10 min. The surviving embryos were transferred into patients on the same day of thawing. RESULTS: Sixty-three embryos were thawed, of which 52 embryos (83%) survived with at least one intact blastomere. Nineteen frozen-thawed embryo transfers were made. The mean embryos per transfer was 2.7. Three pregnancies (16%/transfer) were established. One miscarriage occurred in the eighth week of pregnancy. Two pregnancies went to term and three healthy infants were born. CONCLUSIONS: The present data demonstrate that ultrarapid freezing is a method worth consideration in the area of human embryo freezing.
PURPOSE: We report our experience of freezing human embryos using an ultrarapid freezing method. METHODS: The patients were superovulated. Oocytes were inseminated and cultured in HTF + 10% serum. A maximum of three embryos was transferred and the rest of the embryos were frozen ultrarapidly after a 3-min equilibration period in PB1 + 3.5 M DMSO + 0.25 M sucrose. Embryos were thawed in a 37 degrees C water bath for 6 sec, then cultured in PB1 + 20% serum for 10 min. The surviving embryos were transferred into patients on the same day of thawing. RESULTS: Sixty-three embryos were thawed, of which 52 embryos (83%) survived with at least one intact blastomere. Nineteen frozen-thawed embryo transfers were made. The mean embryos per transfer was 2.7. Three pregnancies (16%/transfer) were established. One miscarriage occurred in the eighth week of pregnancy. Two pregnancies went to term and three healthy infants were born. CONCLUSIONS: The present data demonstrate that ultrarapid freezing is a method worth consideration in the area of human embryo freezing.
Authors: J Mandelbaum; A M Junca; M Plachot; M O Alnot; J Salat-Baroux; S Alvarez; C Tibi; J Cohen; C Debache; L Tesquier Journal: Hum Reprod Date: 1988-01 Impact factor: 6.918
Authors: J Testart; B Lassalle; J Belaisch-Allart; A Hazout; R Forman; J D Rainhorn; R Frydman Journal: Fertil Steril Date: 1986-08 Impact factor: 7.329
Authors: S M Hosseini; M Forouzanfar; M Hajian; V Asgari; P Abedi; L Hosseini; S Ostadhosseini; F Moulavi; M Safahani Langrroodi; H Sadeghi; H Bahramian; Sh Eghbalsaied; Mohammad H Nasr-Esfahani Journal: J Assist Reprod Genet Date: 2009-06-19 Impact factor: 3.412