| Literature DB >> 4020239 |
J Cohen, R F Simons, R G Edwards, C B Fehilly, S B Fishel.
Abstract
Human blastocysts were frozen in Earle's solution containing pyruvate and human serum, using glycerol as cryoprotectant, and stored in liquid nitrogen. Thawed blastocysts were replaced in 11 patients, which resulted in two pregnancies. One blastocyst giving a pregnancy was hatching when replaced. Three parameters appeared to be important for embryo survival and implantation: the interval between ovulation and replacement of the thawed blastocysts, satisfactory embryonic development before freezing, and the stage of blastulation when cooling began.Entities:
Mesh:
Year: 1985 PMID: 4020239 DOI: 10.1007/bf01139337
Source DB: PubMed Journal: J In Vitro Fert Embryo Transf ISSN: 0740-7769