Ultrastructural aspects of the intact titanium implant-bone interface from undecalcified ultrathin sections.

An osseointegrated oral implant with surrounding bone was used for electron microscopical analyses of the implant-bone interface. The bulk metal was removed by sawing and grinding techniques, leaving only the plasma-sprayed titanium coating anchored in mineralized bone. Ultrathin sections were realized from these reduced interface areas and underwent ultrastructural and crystallographic assessments. The microscopical observations showed that ultramicrotomy was suitable for producing such interface sections. Two different, concomitant, interfacial structures were noticed. On the one hand it was possible to observe bone crystals directly apposed on the implant surface; on the other, a granular electron-dense substance was interposed between the plasma-sprayed coating and the bone. The applied technical approach allows one to study the osseointegration process, at high resolution levels, of intact interfaces from complete osseointegrated implants.