PURPOSE: To elucidate the magnetization transfer (MT) effects of major components of hyaline cartilage. MATERIALS AND METHODS: The authors evaluated the MT effect of type II collagen and chondroitin sulfate phantoms (concentrations of 0.6%, 1.2%, 2.4%, 4.8%, 9.6%, and 19.2%), mass-cultured rat osteosarcoma-derived cells, and normal articular cartilage of the knee. RESULTS: The MT effect of collagen yielded a decrease in signal intensity of 10% (1.2% collagen) to 31% (19.2% collagen); the decrease varied linearly with collagen concentration. Chondroitin sulfate did not have a statistically significant MT effect. Cell pellets showed a decrease in signal intensity of 22%-26%, which was greater than that of 4.8% collagen. Normal articular cartilage of the knees showed a decrease in signal intensity of 38%-48%. CONCLUSION: The major role of collagen in MT contrast of hyaline cartilage was confirmed. In addition, a higher cellular contribution to the MT effect was also demonstrated.
PURPOSE: To elucidate the magnetization transfer (MT) effects of major components of hyaline cartilage. MATERIALS AND METHODS: The authors evaluated the MT effect of type II collagen and chondroitin sulfate phantoms (concentrations of 0.6%, 1.2%, 2.4%, 4.8%, 9.6%, and 19.2%), mass-cultured ratosteosarcoma-derived cells, and normal articular cartilage of the knee. RESULTS: The MT effect of collagen yielded a decrease in signal intensity of 10% (1.2% collagen) to 31% (19.2% collagen); the decrease varied linearly with collagen concentration. Chondroitin sulfate did not have a statistically significant MT effect. Cell pellets showed a decrease in signal intensity of 22%-26%, which was greater than that of 4.8% collagen. Normal articular cartilage of the knees showed a decrease in signal intensity of 38%-48%. CONCLUSION: The major role of collagen in MT contrast of hyaline cartilage was confirmed. In addition, a higher cellular contribution to the MT effect was also demonstrated.
Authors: Ingrid E Chesnick; Francis A Avallone; Richard D Leapman; William J Landis; Naomi Eidelman; Kimberlee Potter Journal: Bone Date: 2006-12-15 Impact factor: 4.398
Authors: Vanessa A Lukas; Kenneth W Fishbein; Ping-Chang Lin; Michael Schär; Erika Schneider; Corey P Neu; Richard G Spencer; David A Reiter Journal: J Orthop Res Date: 2015-03-05 Impact factor: 3.494
Authors: Ingrid E Chesnick; Jeffrey T Mason; Anthony A Giuseppetti; Naomi Eidelman; Kimberlee Potter Journal: Biophys J Date: 2008-05-16 Impact factor: 4.033