Literature DB >> 8877138

Investigation of immunofluorescence cross-reactions against Trichinella spiralis by western blot (immunoblot) analysis.

F Robert1, B Weil, N Kassis, J Dupouy-Camet.   

Abstract

Immunofluorescence cross-reactions in Trichinella spiralis serodiagnosis are sometimes difficult to identify. We compared the results of an indirect immunofluorescence assay and the profiles obtained by Western blot (immunoblot) analysis for three groups of patients: 10 T. spiralis-infected patients, 10 patients with autoimmune diseases, and 7 patients with parasitic diseases other than trichinellosis. The degree of immunofluorescence cross-reaction was variable. Western blotting allowed us to differentiate Trichinella infection from other parasitic diseases. In 3 of 10 serum samples from patients with autoimmune diseases, bands which had the same sizes as Trichinella bands were observed, and they could correspond to shared epitopes such as heat shock proteins.

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Year:  1996        PMID： 8877138      PMCID： PMC170409          DOI： 10.1128/cdli.3.5.575-577.1996

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


  18 in total

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Journal:  Immunol Today       Date:  1991-03

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Journal:  Parasite       Date:  1994-06       Impact factor: 3.000

3.  Molecular identity of a major antigen of Schistosoma mansoni which cross-reacts with Trichinella spiralis and Fasciola hepatica.

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Journal:  Parasitology       Date:  1986-02       Impact factor: 3.234

Review 4.  Autoimmunity and Chagas' disease.

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Journal:  Curr Top Microbiol Immunol       Date:  1989       Impact factor: 4.291

5.  Antigenic characteristics of two strains of Trichinella spiralis isolated during the horsemeat-related outbreaks of 1985 in France.

Authors:  J Dupouy-Camet; M E Bougnoux; T Ancelle; R Fagard; J Lapierre
Journal:  Parasitol Res       Date:  1988       Impact factor: 2.289

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Journal:  Bull Soc Pathol Exot Filiales       Date:  1976 Mar-Apr

Review 7.  Heat shock proteins and autoimmunity.

Authors:  J S Gaston
Journal:  Semin Immunol       Date:  1991-01       Impact factor: 11.130

8.  Do parasitic infections break T-cell tolerance and trigger autoimmune disease?

Authors:  M Röcken; E M Shevach
Journal:  Parasitol Today       Date:  1993-10

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Authors:  A O Wozencraft; N A Staines
Journal:  Parasitol Today       Date:  1990-08

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Authors:  D Rahima; R Tarrab-Hazdai; M Blank; R Arnon; Y Shoenfeld
Journal:  Autoimmunity       Date:  1994       Impact factor: 2.815
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  11 in total

Review 1.  New aspects of human trichinellosis: the impact of new Trichinella species.

Authors:  F Bruschi; K D Murrell
Journal:  Postgrad Med J       Date:  2002-01       Impact factor: 2.401

2.  Potential use of Trichinella spiralis antigen for serodiagnosis of human capillariasis philippinensis by immunoblot analysis.

Authors:  Pewpan M Intapan; Wanchai Maleewong; Wattana Sukeepaisarnjaroen; Nimit Morakote
Journal:  Parasitol Res       Date:  2005-12-07       Impact factor: 2.289

3.  Validation of an enzyme-linked immunosorbent assay for diagnosis of human trichinellosis.

Authors:  Maria Angeles Gómez-Morales; Alessandra Ludovisi; Marco Amati; Simona Cherchi; Patrizio Pezzotti; Edoardo Pozio
Journal:  Clin Vaccine Immunol       Date:  2008-09-30

4.  Application and assessment of a dipstick assay in the diagnosis of hydatidosis and trichinosis.

Authors:  Maged M Al-Sherbiny; Abd-Al Magid M K Farrag; Mohamed H Fayad; Mohamed K Makled; Gihan M Tawfeek; Nehad M S Ali
Journal:  Parasitol Res       Date:  2004-04-22       Impact factor: 2.289

5.  Development and evaluation of a Western blot kit for diagnosis of human trichinellosis.

Authors:  Hélène Yera; Shakir Andiva; Catherine Perret; Denis Limonne; Pascal Boireau; Jean Dupouy-Camet
Journal:  Clin Diagn Lab Immunol       Date:  2003-09

6.  Validation of a Western Blot for the detection of anti-Trichinella spp. antibodies in domestic pigs.

Authors:  C F Frey; M E Schuppers; K Nöckler; A Marinculić; E Pozio; U Kihm; B Gottstein
Journal:  Parasitol Res       Date:  2009-01-07       Impact factor: 2.289

Review 7.  Epidemiology, diagnosis, treatment, and control of trichinellosis.

Authors:  Bruno Gottstein; Edoardo Pozio; Karsten Nöckler
Journal:  Clin Microbiol Rev       Date:  2009-01       Impact factor: 26.132

8.  Species-specific antibody responses to the recombinant 53-kilodalton excretory and secretory proteins in mice infected with Trichinella spp.

Authors:  Isao Nagano; Zhiliang Wu; Yuzo Takahashi
Journal:  Clin Vaccine Immunol       Date:  2008-01-09

9.  New insights on serodiagnosis of trichinellosis during window period: early diagnostic antigens from Trichinella spiralis intestinal worms.

Authors:  Zhong-Quan Wang; Ya-Li Shi; Rou-Dan Liu; Peng Jiang; Ya-Yi Guan; Ying-Dan Chen; Jing Cui
Journal:  Infect Dis Poverty       Date:  2017-02-20       Impact factor: 4.520

10.  Indirect versus direct detection methods of Trichinella spp. infection in wild boar (Sus scrofa).

Authors:  Maria Angeles Gómez-Morales; Alessandra Ludovisi; Marco Amati; Ennio Bandino; Gioia Capelli; Franco Corrias; Luca Gelmini; Alberigo Nardi; Cristina Sacchi; Simona Cherchi; Marco Lalle; Edoardo Pozio
Journal:  Parasit Vectors       Date:  2014-04-07       Impact factor: 3.876
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