Literature DB >> 8876182

Silver ion high pressure liquid chromatography provides unprecedented separation of sterols: application to the enzymatic formation of cholesta-5,8-dien-3 beta-ol.

B Ruan1, J Shey, N Gerst, W K Wilson, G J Schroepfer.   

Abstract

We report that silver ion HPLC provides remarkable separations of C27 sterols differing only in the number or location of olefinic double bonds. This technique has been extended to LC-MS, analysis of purified components by GC, GC-MS, and 1H NMR, and to its use on a semipreparative scale. The application of this methodology for the demonstration of the catalysis, by rat liver microsomes, of the conversion of 7-dehydrocholesterol to cholesta-5,8-dien-3 beta-ol is also presented.

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Year:  1996        PMID： 8876182      PMCID： PMC38104          DOI： 10.1073/pnas.93.21.11603

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  56 in total

1.  STUDIES ON BRAIN STEROLS IN NORMAL AND PATHOLOGICAL CONDITIONS.

Authors:  R PAOLETTI; R FUMAGALLI; E GROSSI; P PAOLETTI
Journal:  J Am Oil Chem Soc       Date:  1965-05       Impact factor: 1.849

Review 2.  The biological synthesis of cholesterol.

Authors:  K Bloch
Journal:  Science       Date:  1965-10-01       Impact factor: 47.728

3.  The reversibility of the delta8-cholestenol-delta7-cholestenol isomerase reaction in cholesterol biosynthesis.

Authors:  D C Wilton; A D Rahimtula; M Akhtar
Journal:  Biochem J       Date:  1969-08       Impact factor: 3.857

4.  Serum lathosterol levels in human subjects reflect changes in whole body cholesterol synthesis induced by lovastatin but not dietary cholesterol.

Authors:  W C Duane
Journal:  J Lipid Res       Date:  1995-02       Impact factor: 5.922

5.  Chemical structure of sterols that activate oocyte meiosis.

Authors:  A G Byskov; C Y Andersen; L Nordholm; H Thøgersen; G Xia; O Wassmann; J V Andersen; E Guddal; T Roed
Journal:  Nature       Date:  1995-04-06       Impact factor: 49.962

6.  Markedly increased tissue concentrations of 7-dehydrocholesterol combined with low levels of cholesterol are characteristic of the Smith-Lemli-Opitz syndrome.

Authors:  G S Tint; M Seller; R Hughes-Benzie; A K Batta; S Shefer; D Genest; M Irons; E Elias; G Salen
Journal:  J Lipid Res       Date:  1995-01       Impact factor: 5.922

7.  Inhibitors of sterol synthesis. Submicromolar 14 alpha-ethyl-5 alpha-cholest-7-ene-3 beta, 15 alpha-diol causes a major modification of the sterol composition of CHO-K1 cells and a marked change in cell morphology.

Authors:  A Izumi; F D Pinkerton; S O Nelson; J S Pyrek; P J Neill; J H Smith; G J Schroepfer
Journal:  J Lipid Res       Date:  1994-07       Impact factor: 5.922

8.  Cyclic AMP stimulates efflux of intracellular sterol from cholesterol-loaded cells.

Authors:  B M Hokland; J P Slotte; E L Bierman; J F Oram
Journal:  J Biol Chem       Date:  1993-12-05       Impact factor: 5.157

9.  Studies on the mechanism of the enzymatic conversion of delta 8-cholesten-3 beta-ol to delta 7-cholesten-3 beta-ol.

Authors:  W H Lee; R Kammereck; B N Lutsky; J A McCloskey; G J Schroepfer
Journal:  J Biol Chem       Date:  1969-04-25       Impact factor: 5.157

10.  Defective cholesterol biosynthesis associated with the Smith-Lemli-Opitz syndrome.

Authors:  G S Tint; M Irons; E R Elias; A K Batta; R Frieden; T S Chen; G Salen
Journal:  N Engl J Med       Date:  1994-01-13       Impact factor: 91.245
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  5 in total

1.  Dissecting the role of the golgi complex and lipid rafts in biosynthetic transport of cholesterol to the cell surface.

Authors:  S Heino; S Lusa; P Somerharju; C Ehnholm; V M Olkkonen; E Ikonen
Journal:  Proc Natl Acad Sci U S A       Date:  2000-07-18       Impact factor: 11.205

2.  The orphan nuclear receptor LXRalpha is positively and negatively regulated by distinct products of mevalonate metabolism.

Authors:  B M Forman; B Ruan; J Chen; G J Schroepfer; R M Evans
Journal:  Proc Natl Acad Sci U S A       Date:  1997-09-30       Impact factor: 11.205

3.  Fifty years with bile acids and steroids in health and disease.

Authors:  Jan Sjövall
Journal:  Lipids       Date:  2004-08       Impact factor: 1.880

Review 4.  From molecular fossils of bacterial hopanoids to the formation of isoprene units: discovery and elucidation of the methylerythritol phosphate pathway.

Authors:  Michel Rohmer
Journal:  Lipids       Date:  2008-11-15       Impact factor: 1.880

5.  Test Paper for Colorimetric Inspection of Fatty Acids and Edible Oils.

Authors:  Feng Zhang; Xiaojie Wang; Xu Jie; Weili Wei
Journal:  Sensors (Basel)       Date:  2018-09-27       Impact factor: 3.576
  5 in total

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