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Literature DB >> 887100

Denaturation of mouse satellite and ribosomal DNA during hydroxyapatite thermal chromatography of chromatin.

Abstract

Mouse DNA and chromatin were melted on hydroxyapatite and the denaturation profiles of ribosomal and satellite DNAs were followed by hybridization with their complementary RNAs. Neither ribosomal nor bulk DNA had significantly different melting profiles in chromatin as compared to DNA. However, most of satellite DNA eluted at higher temperature from chromatin than from purified DNA. One explaination for the higher melting temperature of mouse satellite DNA in chromatin suggests that the complex between this particular DNA component and at least some proteins in chromatin is more stable than the average DNA-protein interaction.

Entities: Chemical Species

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Year: 1977 PMID： 887100 DOI： 10.1007/bf00368300

Source DB: PubMed Journal: Mol Biol Rep ISSN： 0301-4851 Impact factor: 2.316

11 in total

1. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

Authors: K BURTON
Journal: Biochem J Date: 1956-02 Impact factor: 3.857

2. Histone-DNA interactions in erythrocyte chromatin.

Authors: G R Reeck
Journal: Arch Biochem Biophys Date: 1976-01 Impact factor: 4.013

3. Studies on interaction between histone V (f2c) and deoxyribonucleic acids.

Authors: J C Hwan; I M Leffak; H J Li; P C Huang; C Mura
Journal: Biochemistry Date: 1975-04-08 Impact factor: 3.162

4. Transcription of the ribosomal RNA genes of an amphibian by the RNA polymerase of a bacterium.

Authors: R H Reeder; D D Brown
Journal: J Mol Biol Date: 1970-07-28 Impact factor: 5.469

5. Fractionation of chromatin by thermal chromatography.

Authors: B L McConaughy; B J McCarthy
Journal: Biochemistry Date: 1972-03-14 Impact factor: 3.162

6. Retention of common nucleotide sequences in the ribosomal deoxyribonucleic acid of eukaryotes and some of their physical characteristics.

Authors: J H Sinclair; D D Brown
Journal: Biochemistry Date: 1971-07-06 Impact factor: 3.162

Denaturation of mouse satellite and ribosomal DNA during hydroxyapatite thermal chromatography of chromatin.

1. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

2. Histone-DNA interactions in erythrocyte chromatin.

3. Studies on interaction between histone V (f2c) and deoxyribonucleic acids.

4. Transcription of the ribosomal RNA genes of an amphibian by the RNA polymerase of a bacterium.

5. Fractionation of chromatin by thermal chromatography.

6. Retention of common nucleotide sequences in the ribosomal deoxyribonucleic acid of eukaryotes and some of their physical characteristics.

7. Isolation and characterization of mouse and guinea pig satellite deoxyribonucleic acids.

8. Composition and structure of chromosomal and amplified ribosomal DNA's of Xenopus laevis.

9. Gene linkage by RNA-DNA hybridization. I. Unique DNA sequences homologous to 4 s RNA, 5 s RNA and ribosomal RNA.

10. Structure studies on chromatin and nucleohistones. Thermal denaturation profiles recorded in the presence of urea.

1. Denaturation of mouse satellite DNA upon melting of chromatin in solution.