Literature DB >> 570242

Denaturation of mouse satellite DNA upon melting of chromatin in solution.

I G Pashev, M M Nencheva, G G Markov.   

Abstract

The denaturation of mouse satellite DNA upon melting of chromatin in solution of low ionic strength has been studied. A procedure for preparation of partially denaturated chromatin was developed which enabled the isolation of double-stranded (non-denatured) DNA sequences according to their thermal stability in chromatin. The content of mouse satellite DNA in these DNA sequences was determined by hybridization with RNA, complementary to satellite DNA in order to find the temperature interval of denaturation of satellite DNA. It was found that the melting temperature of satellite DNA in chromatin was lower than that of the total DNA. The results are discussed in relation to previously reported anomalous behaviour of satellite DNA upon melting of chromatin on hydroxyapatite.

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Year:  1978        PMID： 570242     DOI： 10.1007/bf00777514

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  16 in total

1.  A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

Authors:  K BURTON
Journal:  Biochem J       Date:  1956-02       Impact factor: 3.857

2.  Studies on interaction between histone V (f2c) and deoxyribonucleic acids.

Authors:  J C Hwan; I M Leffak; H J Li; P C Huang; C Mura
Journal:  Biochemistry       Date:  1975-04-08       Impact factor: 3.162

3.  Denaturation of mouse satellite and ribosomal DNA during hydroxyapatite thermal chromatography of chromatin.

Authors:  I G Pashev
Journal:  Mol Biol Rep       Date:  1977-06       Impact factor: 2.316

4.  A nuclease specific for heat-denatured DNA in isolated from a product of Aspergillus oryzae.

Authors:  T Ando
Journal:  Biochim Biophys Acta       Date:  1966-01-18

5.  Transcription of the ribosomal RNA genes of an amphibian by the RNA polymerase of a bacterium.

Authors:  R H Reeder; D D Brown
Journal:  J Mol Biol       Date:  1970-07-28       Impact factor: 5.469

6.  Isolation and characterization of mouse and guinea pig satellite deoxyribonucleic acids.

Authors:  G Corneo; E Ginelli; C Soave; G Bernardi
Journal:  Biochemistry       Date:  1968-12       Impact factor: 3.162

7.  Properties of formaldehyde-treated nucleohistone.

Authors:  D Brutlag; C Schlehuber; J Bonner
Journal:  Biochemistry       Date:  1969-08       Impact factor: 3.162

8.  Absence of short period interspersion of repetitive and non-repetitive sequences in the DNA of Drosophila melanogaster.

Authors:  W R Crain; F C Eden; W R Pearson; E H Davidson; R J Britten
Journal:  Chromosoma       Date:  1976-07-30       Impact factor: 4.316

9.  Hydrocyapatite thermal chromatography of chromatin. Elution pattern of mouse satellite DNA in partially dehistonized and reconstituted chromatin.

Authors:  I G Pashev; G G Markov
Journal:  Int J Biochem       Date:  1978

10.  Histone redistribution and conformational effect on chromatin induced by formaldehyde.

Authors:  I Polacow; L Cabasso; H J Li
Journal:  Biochemistry       Date:  1976-10-19       Impact factor: 3.162
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  1 in total

1.  Protein-DNA crosslinking in reconstituted nucleohistone, nuclei and whole cells by picosecond UV laser irradiation.

Authors:  D Angelov; S I Dimitrov; V R Russanova; E Keskinova; I G Pashev
Journal:  Nucleic Acids Res       Date:  1988-05-25       Impact factor: 16.971
  1 in total

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