| Literature DB >> 8865347 |
S G Reddy1, G Scapin, J S Blanchard.
Abstract
The gene encoding the meso-diaminopimelate dehydrogenase (DAPDH) from Corynebacterium glutamicum was over-expressed and purified to homogeneity. Crystals of the binary DAPDH-NADP+ complex were obtained from solutions of polyethylene glycol 8000, 100 mM sodium cacodylate, pH 6.5, and 150-300 mM Mg(OAc)2. The crystals diffract to 2.2 A, belong to the orthorhombic space group P2(1), and contain two molecules per asymmetric unit.Entities:
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Year: 1996 PMID： 8865347 DOI： 10.1002/prot.12
Source DB: PubMed Journal: Proteins ISSN： 0887-3585