PURPOSE: The present study examines the relationship between size and particle transit across the mucosal barrier of the gastrointestinal tract to other sites of the body. The extent of particle uptake with increasing size, the tissue distribution and cut-off points for 2-20 microns particles is investigated. METHODS: An established fluorescent latex particle-young adult rat model is used and particle numbers in small intestine and mesenteric lymph nodes, 0.5 h post administration, counted by fluorescence microscopy in bulk tissue specimens and cryosections. RESULTS: Bulk tissue analysis provides evidence for the presence of particles of all sizes in the Peyer's patch regions, but only for 2 microns particles in the nodal tissues. Microscopy establishes uptake of both 2 and 6 microns particles in most intestinal and nodal tissue sites and compartments. By contrast, uptake of the larger particles is much reduced. CONCLUSIONS: Although more of the smaller (2 microns) particles are taken up, particularly by epithelial tissues, the 6 microns size appears more efficient in terms of volume translocated to lymph nodes. This could have implications in the therapeutic use of particles for drug and vaccine delivery and for radiation safety.
PURPOSE: The present study examines the relationship between size and particle transit across the mucosal barrier of the gastrointestinal tract to other sites of the body. The extent of particle uptake with increasing size, the tissue distribution and cut-off points for 2-20 microns particles is investigated. METHODS: An established fluorescent latex particle-young adult rat model is used and particle numbers in small intestine and mesenteric lymph nodes, 0.5 h post administration, counted by fluorescence microscopy in bulk tissue specimens and cryosections. RESULTS: Bulk tissue analysis provides evidence for the presence of particles of all sizes in the Peyer's patch regions, but only for 2 microns particles in the nodal tissues. Microscopy establishes uptake of both 2 and 6 microns particles in most intestinal and nodal tissue sites and compartments. By contrast, uptake of the larger particles is much reduced. CONCLUSIONS: Although more of the smaller (2 microns) particles are taken up, particularly by epithelial tissues, the 6 microns size appears more efficient in terms of volume translocated to lymph nodes. This could have implications in the therapeutic use of particles for drug and vaccine delivery and for radiation safety.
Authors: Petra Jackson; Karin Sørig Hougaard; Anne Mette Z Boisen; Nicklas Raun Jacobsen; Keld Alstrup Jensen; Peter Møller; Gunnar Brunborg; Kristine Bjerve Gutzkow; Ole Andersen; Steffen Loft; Ulla Vogel; Håkan Wallin Journal: Nanotoxicology Date: 2011-06-08 Impact factor: 5.913
Authors: Simon Clark; Martin L Cross; Allan Nadian; Julia Vipond; Pinar Court; Ann Williams; R Glyn Hewinson; Frank E Aldwell; Mark A Chambers Journal: Infect Immun Date: 2008-06-02 Impact factor: 3.441
Authors: Janne K Folkmann; Lotte Risom; Nicklas R Jacobsen; Håkan Wallin; Steffen Loft; Peter Møller Journal: Environ Health Perspect Date: 2008-11-12 Impact factor: 9.031