| Literature DB >> 8844426 |
S M Mansor1, V Navaratnam, N Yahaya, N K Nair, W H Wernsdorfer, P H Degen.
Abstract
A rapid and selective high-performance liquid chromatographic assay for determination of a new antimalarial drug (benflumetol, BFL) is described. After extraction with hexane-diethyl ether (70:30, v/v) from plasma, BFL was analysed using a C18 Partisil 10 ODS-3 reversed-phase stainless steel column and a mobile phase of acetonitrile-0.1 M ammonium acetate (90:10, v/v) adjusted to pH 4.9 with ultraviolet detection at 335 nm. The mean recovery of BFL over a concentration range of 50-400 ng/ml was 96.8 +/- 5.2%. The within-day and day-to-day coefficients of variation were 1.8-4.0 and 1.8-4.2%, respectively. The minimum detectable concentration in plasma for BFL was 5 ng/ml with a C.V. of less than 10%. This method was found to be suitable for clinical pharmacokinetic studies.Entities:
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Year: 1996 PMID： 8844426 DOI： 10.1016/0378-4347(96)00086-2
Source DB: PubMed Journal: J Chromatogr B Biomed Appl ISSN： 1572-6495