Literature DB >> 8837480

Comparison of Mycoplasma agalactiae isolates by pulsed field gel electrophoresis, SDS-PAGE and immunoblotting.

S Tola1, G Idini, D Manunta, I Casciano, A M Rocchigiani, A Angioi, G Leori.   

Abstract

We have analyzed 81 isolates of Mycoplasma agalactiae from four different regions of Italy between 1990 and 1995 in order to identify antigenic differences through SDS-PAGE and Western blotting and chromosomal DNA restriction endonuclease cleavage pattern differences. Antigenic variability in M. agalactiae isolates was investigated analyzing hydrophobic membrane protein fractions by immunoblotting using pooled sheep antiserum from naturally infected sheep. Large restriction fragments obtained cleaving genomic DNAs with SmaI, NruI, SalI, XhoI, BssHII and KpnI were analyzed by pulsed field gel electrophoresis. Genetic analysis indicates that isolates are all similar without intraspecific differences. This homogeneity was confirmed by immunoblotting: 80 and 50 kDa antigens are present in all strains analyzed.

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Year:  1996        PMID： 8837480     DOI： 10.1111/j.1574-6968.1996.tb08490.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  10 in total

1.  P48 major surface antigen of Mycoplasma agalactiae is homologous to a malp product of Mycoplasma fermentans and belongs to a selected family of bacterial lipoproteins.

Authors:  S Rosati; S Pozzi; P Robino; B Montinaro; A Conti; M Fadda; M Pittau
Journal:  Infect Immun       Date:  1999-11       Impact factor: 3.441

2.  Characterization of a multigene family undergoing high-frequency DNA rearrangements and coding for abundant variable surface proteins in Mycoplasma agalactiae.

Authors:  M D Glew; L Papazisi; F Poumarat; D Bergonier; R Rosengarten; C Citti
Journal:  Infect Immun       Date:  2000-08       Impact factor: 3.441

3.  Mapping antigenic sites of an immunodominant surface lipoprotein of Mycoplasma agalactiae, AvgC, with the use of synthetic peptides.

Authors:  Antonella Santona; Franco Carta; Peppinetta Fraghí; Franco Turrini
Journal:  Infect Immun       Date:  2002-01       Impact factor: 3.441

4.  Development of a sensitive and specific enzyme-linked immunosorbent assay based on recombinant antigens for rapid detection of antibodies against Mycoplasma agalactiae in sheep.

Authors:  Marisa Fusco; Lucia Corona; Toniangelo Onni; Elisabetta Marras; Carla Longheu; Graziano Idini; Sebastiana Tola
Journal:  Clin Vaccine Immunol       Date:  2007-02-07

5.  Molecular characterization of Mycoplasma agalactiae reveals the presence of an endemic clone in Spain.

Authors:  Jaime Ariza-Miguel; David Rodríguez-Lázaro; Marta Hernández
Journal:  J Clin Microbiol       Date:  2012-12-05       Impact factor: 5.948

6.  Mycoplasma agassizii strain variation and distinct host antibody responses explain differences between enzyme-linked immunosorbent assays and Western blot assays.

Authors:  Lori D Wendland; Paul A Klein; Elliott R Jacobson; Mary B Brown
Journal:  Clin Vaccine Immunol       Date:  2010-09-01

Review 7.  Contagious Agalactia In Sheep And Goats: Current Perspectives.

Authors:  Maryne Jaÿ; Florence Tardy
Journal:  Vet Med (Auckl)       Date:  2019-12-27

8.  Unexpected genetic diversity of Mycoplasma agalactiae caprine isolates from an endemic geographically restricted area of Spain.

Authors:  Christian De la Fe; Joaquín Amores; Florence Tardy; Eveline Sagne; Laurent-Xavier Nouvel; Christine Citti
Journal:  BMC Vet Res       Date:  2012-08-27       Impact factor: 2.741

9.  VNTR analysis reveals unexpected genetic diversity within Mycoplasma agalactiae, the main causative agent of contagious agalactia.

Authors:  Laura McAuliffe; Colin P Churchward; Joanna R Lawes; Guido Loria; Roger D Ayling; Robin Aj Nicholas
Journal:  BMC Microbiol       Date:  2008-11-07       Impact factor: 3.605

Review 10.  Mycoplasma agalactiae, an Etiological Agent of Contagious Agalactia in Small Ruminants: A Review.

Authors:  Amit Kumar; Anu Rahal; Sandip Chakraborty; Amit Kumar Verma; Kuldeep Dhama
Journal:  Vet Med Int       Date:  2014-07-03
  10 in total

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