Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Evaluation of cyclohexenoesculetin-beta-D-galactoside and 8-hydroxyquinoline-beta-D-galactoside as substrates for the detection of beta-galactosidase.

Literature DB >> 8837443

Evaluation of cyclohexenoesculetin-beta-D-galactoside and 8-hydroxyquinoline-beta-D-galactoside as substrates for the detection of beta-galactosidase.

A L James¹, J D Perry, M Ford, L Armstrong, F K Gould.

Abstract

We describe the synthesis of two new substrates for the detection of beta-galactosidase and evaluate their performance in comparison with that of 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal). Of 171 Enterobacteriaceae strains that were able to hydrolyze X-Gal, 166 (97.1%) also hydrolyzed cyclohexenoesculetin-beta-D-galactoside whereas only 96 (56.1%) showed evidence of hydrolysis of 8-hydroxyquinoline-beta-D-galactoside. No false-positive results were observed with either substrate.

Entities: Chemical Gene

Mesh：

Substances：

Year: 1996 PMID： 8837443 PMCID： PMC168196 DOI： 10.1128/aem.62.10.3868-3870.1996

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

9 in total

1. The influence of chemical constitution on antibacterial activity. VI. The bactericidal action of 8-hydroxyquinoline (oxine).

Authors: A ALBERT; M I GIBSON; S D RUBBO
Journal: Br J Exp Pathol Date: 1953-04

Review 2. Fluorogenic and chromogenic substrates used in bacterial diagnostics.

Authors: M Manafi; W Kneifel; S Bascomb
Journal: Microbiol Rev Date: 1991-09

3. The rapid detection of lactose fermentation in paracolon organisms by the demonstration of beta-D-galactosidase.

Authors: G H LOWE
Journal: J Med Lab Technol Date: 1962-01

4. Evaluation of new medium with chromogenic substrates for members of the family Enterobacteriaceae in urine samples.

Authors: H Kodaka; M Ishikawa; M Iwata; F Kashitani; S Mizuochi; K Yamaguchi
Journal: J Clin Microbiol Date: 1995-01 Impact factor: 5.948

5. Rapid detection of total and fecal coliforms in water by enzymatic hydrolysis of 4-methylumbelliferone-beta-D-galactoside.

Authors: J D Berg; L Fiksdal
Journal: Appl Environ Microbiol Date: 1988-08 Impact factor: 4.792

6. Detection of specific bacterial enzymes by high contrast metal chelate formation. Part II. Specific detection of Escherichia coli on multipoint-inoculated plates using 8-hydroxyquinoline-beta-D-glucuronide.

Authors: A L James; P Yeoman
Journal: Zentralbl Bakteriol Mikrobiol Hyg A Date: 1988-01

7. Evaluation of a new agar in Uricult-Trio for rapid detection of Escherichia coli in urine.

Authors: F Dalet; T Segovia
Journal: J Clin Microbiol Date: 1995-05 Impact factor: 5.948

8. Use of fluorescein-di-beta-D-galactopyranoside (FDG) and C12-FDG as substrates for beta-galactosidase detection by flow cytometry in animal, bacterial, and yeast cells.

Authors: A Plovins; A M Alvarez; M Ibañez; M Molina; C Nombela
Journal: Appl Environ Microbiol Date: 1994-12 Impact factor: 4.792

9. Detection of specific bacterial enzymes by high contrast metal chelate formation. Part I. 8-Hydroxyquinoline-beta-D-glucoside, an alternative to aesculin in the differentiation of members of the family Enterobacteriaceae.

Authors: A L James; P Yeoman
Journal: Zentralbl Bakteriol Mikrobiol Hyg A Date: 1987-12

9 in total

7 in total

7. Esculin hydrolysis negative and TcdA-only producing strains of Clostridium (Clostridioides) difficile from the environment in Western Australia.

Authors: Nirajmohan Shivaperumal; Daniel R Knight; Korakrit Imwattana; Grace O Androga; Barbara J Chang; Thomas V Riley
Journal: J Appl Microbiol Date: 2022-03-06 Impact factor: 4.059

7 in total

Evaluation of cyclohexenoesculetin-beta-D-galactoside and 8-hydroxyquinoline-beta-D-galactoside as substrates for the detection of beta-galactosidase.

1. The influence of chemical constitution on antibacterial activity. VI. The bactericidal action of 8-hydroxyquinoline (oxine).

Review 2. Fluorogenic and chromogenic substrates used in bacterial diagnostics.

3. The rapid detection of lactose fermentation in paracolon organisms by the demonstration of beta-D-galactosidase.

4. Evaluation of new medium with chromogenic substrates for members of the family Enterobacteriaceae in urine samples.

5. Rapid detection of total and fecal coliforms in water by enzymatic hydrolysis of 4-methylumbelliferone-beta-D-galactoside.

6. Detection of specific bacterial enzymes by high contrast metal chelate formation. Part II. Specific detection of Escherichia coli on multipoint-inoculated plates using 8-hydroxyquinoline-beta-D-glucuronide.

7. Evaluation of a new agar in Uricult-Trio for rapid detection of Escherichia coli in urine.

8. Use of fluorescein-di-beta-D-galactopyranoside (FDG) and C12-FDG as substrates for beta-galactosidase detection by flow cytometry in animal, bacterial, and yeast cells.

9. Detection of specific bacterial enzymes by high contrast metal chelate formation. Part I. 8-Hydroxyquinoline-beta-D-glucoside, an alternative to aesculin in the differentiation of members of the family Enterobacteriaceae.

1. Evaluation of p-naphtholbenzein-beta-D-galactoside as a substrate for bacterial beta-galactosidase.

2. Fluorogenic selective and differential medium for isolation of Enterobacter sakazakii.

Review 3. Chromo-fluorogenic probes for β-galactosidase detection.

Review 4. Molecular Relationships in Biofilm Formation and the Biosynthesis of Exoproducts in Pseudoalteromonas spp.

5. ABC medium, a new chromogenic agar for selective isolation of Salmonella spp.

Review 6. Modified Enzyme Substrates for the Detection of Bacteria: A Review.

7. Esculin hydrolysis negative and TcdA-only producing strains of Clostridium (Clostridioides) difficile from the environment in Western Australia.