Literature DB >> 11097942

Evaluation of p-naphtholbenzein-beta-D-galactoside as a substrate for bacterial beta-galactosidase.

A L James1, K F Chilvers, J D Perry, L Armstrong, F K Gould.   

Abstract

We describe the synthesis of a new substrate for the detection of beta-galactosidase and evaluate its performance in comparison with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) and cyclohexenoesculetinbeta-D-galactoside (CHE-Gal). Of 206 Enterobacteriaceae strains able to hydrolyze X-Gal, 194 (94.2%) hydrolyzed CHE-Gal and 192 (93.2%) hydrolyzed p-naphtholbenzein-beta-D-galactoside (PNB-Gal). We conclude that PNB-Gal is an effective substrate for the detection of beta-galactosidase.

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Year:  2000        PMID： 11097942      PMCID： PMC92496          DOI： 10.1128/AEM.66.12.5521-5523.2000

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  8 in total

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Journal:  Eur J Clin Microbiol Infect Dis       Date:  1999-11       Impact factor: 3.267

4.  Evaluation of new medium with chromogenic substrates for members of the family Enterobacteriaceae in urine samples.

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Journal:  J Clin Microbiol       Date:  1995-01       Impact factor: 5.948

5.  Rapid detection of total and fecal coliforms in water by enzymatic hydrolysis of 4-methylumbelliferone-beta-D-galactoside.

Authors:  J D Berg; L Fiksdal
Journal:  Appl Environ Microbiol       Date:  1988-08       Impact factor: 4.792

6.  Evaluation of cyclohexenoesculetin-beta-D-galactoside and 8-hydroxyquinoline-beta-D-galactoside as substrates for the detection of beta-galactosidase.

Authors:  A L James; J D Perry; M Ford; L Armstrong; F K Gould
Journal:  Appl Environ Microbiol       Date:  1996-10       Impact factor: 4.792

7.  A new plate medium for rapid presumptive identification and differentiation of Enterobacteriaceae.

Authors:  M Manafi; M L Rotter
Journal:  Int J Food Microbiol       Date:  1991-11       Impact factor: 5.277

8.  Use of fluorescein-di-beta-D-galactopyranoside (FDG) and C12-FDG as substrates for beta-galactosidase detection by flow cytometry in animal, bacterial, and yeast cells.

Authors:  A Plovins; A M Alvarez; M Ibañez; M Molina; C Nombela
Journal:  Appl Environ Microbiol       Date:  1994-12       Impact factor: 4.792
  8 in total

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