| Literature DB >> 8836200 |
K Kobayashi1, T Ohye, I Pastan, T Nagatsu.
Abstract
Immunotoxoin-mediated cell targeting (IMCT) is a technique for conditionally ablating specific cell types based on the cytotoxic activity of a recombinant immunotoxin anti-Tac (Fv)-PE40. To examine the feasibility of this technique for the negative selection in mouse embryonic stem (ES) cells, we investigated the responsiveness of cells expressing human interleukin-2 receptor alpha subunit to anti-Tac(Fv)-PE40. The immunotoxin treatment efficiently eliminated only ES cells bearing the receptor as a consequence of the target specificity of anti-Tac(Fv)-PE40, indicating that IMCT can be used as a novel strategy for positive and negative selection to enrich ES cell clones with a targeted mutation.Entities:
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Year: 1996 PMID： 8836200 PMCID： PMC146128 DOI： 10.1093/nar/24.18.3653
Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 19.160