Literature DB >> 3194019

Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes.

S L Mansour1, K R Thomas, M R Capecchi.   

Abstract

Gene targeting--homologous recombination of DNA sequences residing in the chromosome with newly introduced DNA sequences--in mouse embryo-derived stem cells promises to provide a means to generate mice of any desired genotype. We describe a positive nd negative selection procedure that enriches 2,000-fold for those cells that contain a targeted mutation. The procedure was applied to the isolation of hprt- and int-2- mutants, but it should be applicable to any gene.

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Year:  1988        PMID： 3194019     DOI： 10.1038/336348a0

Source DB:  PubMed          Journal:  Nature        ISSN： 0028-0836            Impact factor:   49.962


  523 in total

1.  The mechanism of mammalian gene replacement is consistent with the formation of long regions of heteroduplex DNA associated with two crossing-over events.

Authors:  J Li; L R Read; M D Baker
Journal:  Mol Cell Biol       Date:  2001-01       Impact factor: 4.272

2.  Simultaneous Cre catalyzed recombination of two alleles to restore neomycin sensitivity and facilitate homozygous mutations.

Authors:  D S Milstone; G Bradwin; R M Mortensen
Journal:  Nucleic Acids Res       Date:  1999-08-01       Impact factor: 16.971

3.  Enrichment and efficient screening of ES cells containing a targeted mutation: the use of DT-A gene with the polyadenylation signal as a negative selection maker.

Authors:  Y Yanagawa; T Kobayashi; M Ohnishi; T Kobayashi; S Tamura; T Tsuzuki; M Sanbo; T Yagi; F Tashiro; J Miyazaki
Journal:  Transgenic Res       Date:  1999-06       Impact factor: 2.788

4.  How close are we to implementing gene targeting in animals other than the mouse?

Authors:  M R Capecchi
Journal:  Proc Natl Acad Sci U S A       Date:  2000-02-01       Impact factor: 11.205

5.  Notch signaling is essential for vascular morphogenesis in mice.

Authors:  L T Krebs; Y Xue; C R Norton; J R Shutter; M Maguire; J P Sundberg; D Gallahan; V Closson; J Kitajewski; R Callahan; G H Smith; K L Stark; T Gridley
Journal:  Genes Dev       Date:  2000-06-01       Impact factor: 11.361

Review 6.  Modification and repression of genes expressed in the mammary gland using gene targeting and other technologies.

Authors:  J L Vilotte; P L'Huillier; J C Mercier
Journal:  J Mammary Gland Biol Neoplasia       Date:  1998-07       Impact factor: 2.673

7.  Efficient repetitive alteration of the mouse Huntington's disease gene by management of background in the tag and exchange gene targeting strategy.

Authors:  J A Cearley; P J Detloff
Journal:  Transgenic Res       Date:  2001-12       Impact factor: 2.788

Review 8.  Manipulating the mammalian genome by homologous recombination.

Authors:  K M Vasquez; K Marburger; Z Intody; J H Wilson
Journal:  Proc Natl Acad Sci U S A       Date:  2001-07-17       Impact factor: 11.205

9.  Genetic dissection of cadherin function during nephrogenesis.

Authors:  Ulf Dahl; Anders Sjödin; Lionel Larue; Glenn L Radice; Stefan Cajander; Masatoshi Takeichi; Rolf Kemler; Henrik Semb
Journal:  Mol Cell Biol       Date:  2002-03       Impact factor: 4.272

10.  Genetic ablation of phosphatidylinositol transfer protein function in murine embryonic stem cells.

Authors:  James G Alb; Scott E Phillips; Kathleen Rostand; Xiaoxia Cui; Jef Pinxteren; Laura Cotlin; Timothy Manning; Shuling Guo; John D York; Harald Sontheimer; James F Collawn; Vytas A Bankaitis
Journal:  Mol Biol Cell       Date:  2002-03       Impact factor: 4.138
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