Literature DB >> 8809023

Recombinant apoaequorin acting as a pseudo-luciferase reports micromolar changes in the endoplasmic reticulum free Ca2+ of intact cells.

J M Kendall1, M N Badminton, G B Sala-Newby, A K Campbell, C M Rembold.   

Abstract

We describe a novel method to monitor the endoplasmic reticulum (ER) free Ca2+ in intact cells. Continuous perfusion of HeLa cells, expressing ER-targeted apoaequorin, with coelenterazine allowed the apoprotein to act as a pseudo-luciferase capable of reporting free Ca2+ from 0.1-100 microM. In intact HeLa cells, addition of ionomycin increased apoaequorin-generated light by 91%, indicating that resting ER free Ca2+ was approx. 2 microM. Agonist stimulation decreased the ER apoaequorin signal and proportionally increased cytosolic free Ca2+ consistent with agonist-induced release of Ca2+ from the ER.

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Year:  1996        PMID： 8809023      PMCID： PMC1217633          DOI： 10.1042/bj3180383

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  23 in total

1.  A C-terminal proline is required for bioluminescence of the Ca(2+)-binding photoprotein, aequorin.

Authors:  M Nomura; S Inouye; Y Ohmiya; F I Tsuji
Journal:  FEBS Lett       Date:  1991-12-16       Impact factor: 4.124

2.  Aequorin targeted to the endoplasmic reticulum reveals heterogeneity in luminal Ca++ concentration and reports agonist- or IP3-induced release of Ca++.

Authors:  D Button; A Eidsath
Journal:  Mol Biol Cell       Date:  1996-03       Impact factor: 4.138

3.  Technique for in situ measurement of calcium in intracellular inositol 1,4,5-trisphosphate-sensitive stores using the fluorescent indicator mag-fura-2.

Authors:  A M Hofer; T E Machen
Journal:  Proc Natl Acad Sci U S A       Date:  1993-04-01       Impact factor: 11.205

Review 4.  Inositol trisphosphate and calcium signalling.

Authors:  M J Berridge
Journal:  Nature       Date:  1993-01-28       Impact factor: 49.962

Review 5.  Calcium signaling.

Authors:  D E Clapham
Journal:  Cell       Date:  1995-01-27       Impact factor: 41.582

6.  Ca2+ oscillations in neutrophils triggered by immune complexes result from Ca2+ influx.

Authors:  E V Davies; A K Campbell; M B Hallett
Journal:  Immunology       Date:  1994-05       Impact factor: 7.397

7.  Changes in free calcium in the endoplasmic reticulum of living cells detected using targeted aequorin.

Authors:  J M Kendall; M N Badminton; R L Dormer; A K Campbell
Journal:  Anal Biochem       Date:  1994-08-15       Impact factor: 3.365

8.  Heterogeneity of channel density in inositol-1,4,5-trisphosphate-sensitive Ca2+ stores.

Authors:  K Hirose; M Iino
Journal:  Nature       Date:  1994 Dec 22-29       Impact factor: 49.962

9.  Cyclic Ca2+ changes in intracellular stores of gonadotropes during gonadotropin-releasing hormone-stimulated Ca2+ oscillations.

Authors:  F W Tse; A Tse; B Hille
Journal:  Proc Natl Acad Sci U S A       Date:  1994-10-11       Impact factor: 11.205

10.  Cause of spectral variation in the luminescence of semisynthetic aequorins.

Authors:  O Shimomura
Journal:  Biochem J       Date:  1995-03-01       Impact factor: 3.857
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  4 in total

1.  Measurement of proteases using chemiluminescence-resonance-energy-transfer chimaeras between green fluorescent protein and aequorin.

Authors:  J P Waud; A Bermúdez Fajardo; T Sudhaharan; A R Trimby; J Jeffery; A Jones; A K Campbell
Journal:  Biochem J       Date:  2001-08-01       Impact factor: 3.857

2.  All three Ca2+-binding loops of photoproteins bind calcium ions: the crystal structures of calcium-loaded apo-aequorin and apo-obelin.

Authors:  Lu Deng; Eugene S Vysotski; Svetlana V Markova; Zhi-Jie Liu; John Lee; John Rose; Bi-Cheng Wang
Journal:  Protein Sci       Date:  2005-02-02       Impact factor: 6.725

3.  Membrane proximal lysosomes are the major vesicles responsible for calcium-dependent exocytosis in nonsecretory cells.

Authors:  Jyoti K Jaiswal; Norma W Andrews; Sanford M Simon
Journal:  J Cell Biol       Date:  2002-11-18       Impact factor: 10.539

4.  Ca2+ homeostasis in the endoplasmic reticulum: coexistence of high and low [Ca2+] subcompartments in intact HeLa cells.

Authors:  M Montero; J Alvarez; W J Scheenen; R Rizzuto; J Meldolesi; T Pozzan
Journal:  J Cell Biol       Date:  1997-11-03       Impact factor: 10.539
  4 in total

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