Literature DB >> 8807802

Pulsed-field gel electrophoresis indicates genotypic heterogeneity among Campylobacter upsaliensis strains.

B Bourke1, P M Sherman, D Woodward, H Lior, V L Chan.   

Abstract

To determine the genomic relatedness among a selection of animal and human Campylobacter upsaliensis isolates, macrorestriction profiles were generated for 20 C. upsaliensis strains, among 7 serogroups, using pulsed-field gel electrophoresis (PFGE). XhoI, SalI and SacII restriction enzyme profiles indicated genomic heterogeneity among strains. Using XhoI and SacII restriction enzyme digestion, genomic similarities between some pairs of strains were Lior serogroup specific. The genomic sizes of these isolates varied from 1.74 to 2.09 Mb. These results demonstrate molecular heterogeneity of this species similar to that found among Helicobacter pylori isolates. Among C. upsaliensis strains, PFGE is highly discriminatory and should prove a useful molecular typing method for epidemiological purposes.

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Year:  1996        PMID： 8807802     DOI： 10.1111/j.1574-6968.1996.tb08461.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  7 in total

Review 1.  Genotyping of Campylobacter spp.

Authors:  T M Wassenaar; D G Newell
Journal:  Appl Environ Microbiol       Date:  2000-01       Impact factor: 4.792

2.  Rapid separation of large DNA fragments.

Authors:  S E Peltek; L L Frumin; V V Chasovskikh
Journal:  Dokl Biochem Biophys       Date:  2005 Jul-Aug       Impact factor: 0.788

3.  Speciation of Campylobacter coli, C. jejuni, C. helveticus, C. lari, C. sputorum, and C. upsaliensis by matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Authors:  Robert E Mandrell; Leslie A Harden; Anna Bates; William G Miller; William F Haddon; Clifton K Fagerquist
Journal:  Appl Environ Microbiol       Date:  2005-10       Impact factor: 4.792

4.  Identification of Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, arcobacter butzleri, and A. butzleri-like species based on the glyA gene.

Authors:  S T Al Rashid; I Dakuna; H Louie; D Ng; P Vandamme; W Johnson; V L Chan
Journal:  J Clin Microbiol       Date:  2000-04       Impact factor: 5.948

5.  Genomic heterogeneity and O-antigenic diversity of Campylobacter upsaliensis and Campylobacter helveticus strains isolated from dogs and cats in Germany.

Authors:  I Moser; B Rieksneuwöhner; P Lentzsch; P Schwerk; L H Wieler
Journal:  J Clin Microbiol       Date:  2001-07       Impact factor: 5.948

6.  High-resolution genotyping of Campylobacter upsaliensis strains originating from three continents.

Authors:  P Lentzsch; B Rieksneuwöhner; L H Wieler; H Hotzel; I Moser
Journal:  J Clin Microbiol       Date:  2004-08       Impact factor: 5.948

Review 7.  Campylobacter upsaliensis: waiting in the wings.

Authors:  B Bourke; V L Chan; P Sherman
Journal:  Clin Microbiol Rev       Date:  1998-07       Impact factor: 26.132
  7 in total

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