Literature DB >> 879773

Production of antibody against aflatoxin B1.

F S Chu, I Ueno.   

Abstract

Antibody against aflatoxin B1 was obtained after one multiple-site injection of bovine serum albumin-aflatoxin B1 conjugate into rabbits. The antibody has greatest binding efficiency for aflatoxin B1, less efficiency for B2, G1, and Q1, and least for aflatoxicol, G2, and M1. Sterigmatocystin, coumarin, and 4-hydroxycoumarin did not give a cross-reaction with the antibody. The sensitivity of the binding assay for detection of aflatoxin B1 is in the range of 0.2 to 2.0 ng per 0.5-ml sample. Detailed methods for the preparation of the conjugate, production of immune serum, and methods for antibody titer determination are described.

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Year:  1977        PMID： 879773      PMCID： PMC170837          DOI： 10.1128/aem.33.5.1125-1128.1977

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  7 in total

Review 1.  Mycotoxins.

Authors:  G N Wogan
Journal:  Annu Rev Pharmacol       Date:  1975       Impact factor: 13.820

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  Production of antibody against ochratoxin A.

Authors:  F S Chu; F C Chang; R D Hinsdill
Journal:  Appl Environ Microbiol       Date:  1976-06       Impact factor: 4.792

Review 4.  Analytical methods for mycotoxins.

Authors:  L Stoloff
Journal:  Clin Toxicol       Date:  1972       Impact factor: 4.467

5.  Chromatography of crude aflatoxins on adsorbosil-5.

Authors:  F S Chu
Journal:  J Assoc Off Anal Chem       Date:  1971-11

6.  Aflatoxin B; specific antibodies and their use in radioimmunoassay.

Authors:  J J Langone; H Van Vunakis
Journal:  J Natl Cancer Inst       Date:  1976-03       Impact factor: 13.506

7.  Preparation of oestriol-6-(O-carboxymethyl) oxime and oestrone-6-(carboxymethyl) oxime-bovine serum albumin conjugates.

Authors:  P D Dean; P H Rowe; D Exley
Journal:  Steroids Lipids Res       Date:  1972
  7 in total
  21 in total

1.  Direct and indirect competitive monoclonal antibody-based ELISA of Aflatoxin B1 in groundnut.

Authors:  N Ramakrishna; V K Mehan
Journal:  Mycotoxin Res       Date:  1993-03       Impact factor: 3.833

2.  Immunochemical assay applied to mycotoxin biosynthesis: ELISA comparison of sterigmatocystin production by Aspergillus versicolor and Aspergillus nidulans.

Authors:  D H Chung; M M Abouzied; J J Pestka
Journal:  Mycopathologia       Date:  1989-09       Impact factor: 2.574

3.  Production and characterization of antibody against aflatoxin M1.

Authors:  W O Harder; F S Chu
Journal:  Experientia       Date:  1979-08-15

4.  Analytical methods for aflatoxins in corn and peanuts.

Authors:  D M Wilson
Journal:  Arch Environ Contam Toxicol       Date:  1989 May-Jun       Impact factor: 2.804

5.  Production and characterization of antibody against aflatoxin Q1.

Authors:  T S Fan; G S Zhang; F S Chu
Journal:  Appl Environ Microbiol       Date:  1984-03       Impact factor: 4.792

6.  Production of antibody against T-2 toxin.

Authors:  F S Chu; S Grossman; R D Wei; C J Mirocha
Journal:  Appl Environ Microbiol       Date:  1979-01       Impact factor: 4.792

7.  Isolation of alpaca anti-idiotypic heavy-chain single-domain antibody for the aflatoxin immunoassay.

Authors:  Yanru Wang; Peiwu Li; Zuzana Majkova; Candace R S Bever; Hee Joo Kim; Qi Zhang; Julie E Dechant; Shirley J Gee; Bruce D Hammock
Journal:  Anal Chem       Date:  2013-08-21       Impact factor: 6.986

8.  Production and characterization of polyclonal and monoclonal antibodies against Aflatoxin B1 oxime-BSA in an enzyme-linked immunosorbent assay.

Authors:  C Ward; A Wilkinson; S Bramham; H Lee; H S Chan; G Butcher; A Hutchings; M Morgan
Journal:  Mycotoxin Res       Date:  1990-09       Impact factor: 3.833

9.  A monoclonal antibody-based enzyme-linked immunosorbent assay of aflatoxin B1 in peanut products.

Authors:  O Kawamura; S Nagayama; S Sato; K Ohtani; I Ueno; Y Ueno
Journal:  Mycotoxin Res       Date:  1988-09       Impact factor: 3.833

10.  Novel aflatoxin derivatives and protein conjugates.

Authors:  Christian Cervino; Dietmar Knopp; Michael G Weller; Reinhard Niessner
Journal:  Molecules       Date:  2007-03-27       Impact factor: 4.411
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