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Literature DB >> 573214

Production and characterization of antibody against aflatoxin M1.

Abstract

Antibody against aflatoxin M1 was obtained after immunization of rabbits with bovine serum albumin-afla M1 oxime conjugate. The antibody has greatest binding efficiency for afla M1, and was less efficient for afla B1. Cross-reaction of antibody with aflatoxin Q1, aflatoxicol, and aflatoxin B2a was weak. Aflatoxin B2, G1, and G2 and afla B1-guanine adducts showed almost no cross-reaction with the antibody. The sensitivity of the binding assay for aflatoxin M1 detection is in the range of 1-10 ng per assay. Detailed methods for the preparation of the conjugate, production of immune serum, and methods for antibody determination are described.

Entities: Chemical Species

Mesh：

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Year: 1979 PMID： 573214 DOI： 10.1007/bf01949970

Source DB: PubMed Journal: Experientia ISSN： 0014-4754

16 in total

8. Efficient Conjugation of Aflatoxin M1 With Bovine Serum Albumin through Aflatoxin M1-(O-carboxymethyl) Oxime and Production of Anti-aflatoxin M1 Antibodies.

Authors: Fatemeh Khademi; Masoud Mohammadi; Amir Kiani; Reza Haji Hosseini Baghdadabadi; Shahram Parvaneh; Ali Mostafaie
Journal: Jundishapur J Microbiol Date: 2015-04-18 Impact factor: 0.747

8 in total

Production and characterization of antibody against aflatoxin M1.

Review 1. The role of aflatoxin metabolism in its toxic lesion.

2. Determination of aflatoxin M1 in milk at the parts per trillion level.

3. Preparation and characterization of aflatox-n B1-1-(O-carboxymethyl) oxime.

4. Production of antibody against aflatoxin B1.

5. Production of antibody against ochratoxin A.

6. Collaborative study of methods for the determination and chemical confirmation of aflatoxin M1 in dairy products.

7. [On the presence and the content of aflatoxin M in commercial cheese samples (author's transl)].

8. Reverse phase analytical and preparative high pressure liquid chromatography of aflatoxins.

9. Production of antibody against T-2 toxin.

10. [On the presence and the content of aflatoxin M1 in milk shipped to a dairy plant (author's transl)].

1. Production and characterization of antibody against aflatoxin Q1.

2. Production and characterization of monoclonal antibodies against aflatoxin M1.

3. Quantitation of aflatoxin B1 and aflatoxin B1 antibody by an enzyme-linked immunosorbent microassay.

4. Production and characterization of antibodies cross-reactive with major aflatoxins.

5. Comparison of antibody production against aflatoxin B1 in goats and rabbits.

6. Kinetics of transformation of aflatoxin B1 into aflatoxin M1 in lactating mouse: an ELISA analysis.

7. Production and characterization of aflatoxin B2a antiserum.

8. Efficient Conjugation of Aflatoxin M1 With Bovine Serum Albumin through Aflatoxin M1-(O-carboxymethyl) Oxime and Production of Anti-aflatoxin M1 Antibodies.