Literature DB >> 8796351

Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content.

N Baskaran1, R P Kandpal, A K Bhargava, M W Glynn, A Bale, S M Weissman.   

Abstract

A PCR method for uniform amplification of a mixture of DNA templates differing in GC content is described using the two enzyme approach (Klentaq1 and Pfu DNA polymerase) and a combination of DMSO and betaine. This method was applied to amplify the CGG repeat region from the fragile X region.

Entities:  

Mesh：

Substances：

Year:  1996        PMID： 8796351     DOI： 10.1101/gr.6.7.633

Source DB:  PubMed          Journal:  Genome Res        ISSN： 1088-9051            Impact factor:   9.043


  42 in total

1.  Rapid discrimination of monkey B virus from human herpes simplex viruses by PCR in the presence of betaine.

Authors:  M Hirano; S Nakamura; M Okada; M Ueda; R Mukai
Journal:  J Clin Microbiol       Date:  2000-03       Impact factor: 5.948

2.  New specificity and yield enhancer of polymerase chain reactions.

Authors:  M Kovárová; P Dráber
Journal:  Nucleic Acids Res       Date:  2000-07-01       Impact factor: 16.971

3.  Loop-mediated isothermal amplification of DNA.

Authors:  T Notomi; H Okayama; H Masubuchi; T Yonekawa; K Watanabe; N Amino; T Hase
Journal:  Nucleic Acids Res       Date:  2000-06-15       Impact factor: 16.971

4.  A comprehensive analysis of allelic methylation status of CpG islands on human chromosome 21q.

Authors:  Yoichi Yamada; Hidemi Watanabe; Fumihito Miura; Hidenobu Soejima; Michiko Uchiyama; Tsuyoshi Iwasaka; Tsunehiro Mukai; Yoshiyuki Sakaki; Takashi Ito
Journal:  Genome Res       Date:  2004-02       Impact factor: 9.043

5.  Regionalized GC content of template DNA as a predictor of PCR success.

Authors:  Yair Benita; Ronald S Oosting; Martin C Lok; Michael J Wise; Ian Humphery-Smith
Journal:  Nucleic Acids Res       Date:  2003-08-15       Impact factor: 16.971

6.  Amplification of GC-rich DNA for high-throughput family-based genetic studies.

Authors:  Sadaf Naz; Amara Fatima
Journal:  Mol Biotechnol       Date:  2013-03       Impact factor: 2.695

7.  MARA: a novel approach for highly multiplexed locus-specific SNP genotyping using high-density DNA oligonucleotide arrays.

Authors:  Michael H Shapero; Jane Zhang; Ann Loraine; Weiwei Liu; Xiaojun Di; Guoying Liu; Keith W Jones
Journal:  Nucleic Acids Res       Date:  2004-12-15       Impact factor: 16.971

8.  Single molecule measurement of the "speed limit" of DNA polymerase.

Authors:  Jerrod J Schwartz; Stephen R Quake
Journal:  Proc Natl Acad Sci U S A       Date:  2009-11-11       Impact factor: 11.205

9.  Probing the transcription mechanisms of reovirus cores with molecules that alter RNA duplex stability.

Authors:  Alexander A Demidenko; Max L Nibert
Journal:  J Virol       Date:  2009-03-18       Impact factor: 5.103

10.  Identification and characterization of differentially expressed genes from Fagus sylvatica roots after infection with Phytophthora citricola.

Authors:  Katja Schlink
Journal:  Plant Cell Rep       Date:  2009-03-17       Impact factor: 4.570
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.