Literature DB >> 8794372

A p6Pol-protease fusion protein is present in mature particles of human immunodeficiency virus type 1.

N Almog1, R Roller, G Arad, L Passi-Even, M A Wainberg, M Kotler.   

Abstract

Human immunodeficiency virus type 1 (HIV-1) protease (PR) and p6(Pol) are translated as part of the Gag-Pol polyprotein after a ribosomal frameshift. PR is essential to virus replication and is responsible for cleaving Gag and Gag-Pol precursors, but the role of p6(Pol) in HIV-1 infection is poorly understood. Here, we report that (i) PR is present in mature HIV-1 virions primarily as a p6(Pol)-PR fusion protein; (ii) HIV-1 PR cleaves viral precursor proteins expressed in bacterial cells at the Phe-Leu bond (positions 1639 to 1642) located at the junction of the NC and p6(Pol) proteins, releasing the p6(Pol)-PR fusion protein; and (iii) purified p6(Pol)-PR fusion protein undergoes autocleavage in vitro at at least three sites.

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Year:  1996        PMID： 8794372      PMCID： PMC190778     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  29 in total

1.  Mutational analysis of human immunodeficiency virus type 1 protease suggests functional homology with aspartic proteinases.

Authors:  D D Loeb; C A Hutchison; M H Edgell; W G Farmerie; R Swanstrom
Journal:  J Virol       Date:  1989-01       Impact factor: 5.103

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Authors:  K von der Helm
Journal:  Proc Natl Acad Sci U S A       Date:  1977-03       Impact factor: 11.205

Review 3.  Viral proteinases.

Authors:  H G Kräusslich; E Wimmer
Journal:  Annu Rev Biochem       Date:  1988       Impact factor: 23.643

4.  Characterization of ribosomal frameshifting in HIV-1 gag-pol expression.

Authors:  T Jacks; M D Power; F R Masiarz; P A Luciw; P J Barr; H E Varmus
Journal:  Nature       Date:  1988-01-21       Impact factor: 49.962

5.  Genetic locus, primary structure, and chemical synthesis of human immunodeficiency virus protease.

Authors:  T D Copeland; S Oroszlan
Journal:  Gene Anal Tech       Date:  1988 Nov-Dec

6.  Human immunodeficiency virus protease expressed in Escherichia coli exhibits autoprocessing and specific maturation of the gag precursor.

Authors:  C Debouck; J G Gorniak; J E Strickler; T D Meek; B W Metcalf; M Rosenberg
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

7.  In situ processing of a retroviral nucleocapsid protein by the viral proteinase.

Authors:  M M Roberts; T D Copeland; S Oroszlan
Journal:  Protein Eng       Date:  1991-08

8.  Murine leukemia virus maturation: protease region required for conversion from "immature" to "mature" core form and for virus infectivity.

Authors:  I Katoh; Y Yoshinaka; A Rein; M Shibuya; T Odaka; S Oroszlan
Journal:  Virology       Date:  1985-09       Impact factor: 3.616

9.  An 11-kDa form of human immunodeficiency virus protease expressed in Escherichia coli is sufficient for enzymatic activity.

Authors:  M C Graves; J J Lim; E P Heimer; R A Kramer
Journal:  Proc Natl Acad Sci U S A       Date:  1988-04       Impact factor: 11.205

10.  Active human immunodeficiency virus protease is required for viral infectivity.

Authors:  N E Kohl; E A Emini; W A Schleif; L J Davis; J C Heimbach; R A Dixon; E M Scolnick; I S Sigal
Journal:  Proc Natl Acad Sci U S A       Date:  1988-07       Impact factor: 11.205
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  10 in total

1.  The dimer interfaces of protease and extra-protease domains influence the activation of protease and the specificity of GagPol cleavage.

Authors:  Steven C Pettit; Sergei Gulnik; Lori Everitt; Andrew H Kaplan
Journal:  J Virol       Date:  2003-01       Impact factor: 5.103

2.  Human immunodeficiency virus type 1 (HIV-1) protein Vif inhibits the activity of HIV-1 protease in bacteria and in vitro.

Authors:  M Kotler; M Simm; Y S Zhao; P Sova; W Chao; S F Ohnona; R Roller; C Krachmarov; M J Potash; D J Volsky
Journal:  J Virol       Date:  1997-08       Impact factor: 5.103

3.  Uncoupling human immunodeficiency virus type 1 Gag and Pol reading frames: role of the transframe protein p6* in viral replication.

Authors:  Andreas Leiherer; Christine Ludwig; Ralf Wagner
Journal:  J Virol       Date:  2009-04-29       Impact factor: 5.103

4.  Identification of folding preferences of cleavage junctions of HIV-1 precursor proteins for regulation of cleavability.

Authors:  Hirotaka Ode; Masaru Yokoyama; Tadahito Kanda; Hironori Sato
Journal:  J Mol Model       Date:  2010-05-18       Impact factor: 1.810

5.  Proline residues within spacer peptide p1 are important for human immunodeficiency virus type 1 infectivity, protein processing, and genomic RNA dimer stability.

Authors:  Melissa K Hill; Miranda Shehu-Xhilaga; Suzanne M Crowe; Johnson Mak
Journal:  J Virol       Date:  2002-11       Impact factor: 5.103

6.  Cleavage of human immunodeficiency virus type 1 proteinase from the N-terminally adjacent p6* protein is essential for efficient Gag polyprotein processing and viral infectivity.

Authors:  U Tessmer; H G Kräusslich
Journal:  J Virol       Date:  1998-04       Impact factor: 5.103

7.  Human immunodeficiency virus (HIV) type 1 transframe protein can restore activity to a dimerization-deficient HIV protease variant.

Authors:  Nathalie Dautin; Gouzel Karimova; Daniel Ladant
Journal:  J Virol       Date:  2003-08       Impact factor: 5.103

8.  Importance of protease cleavage sites within and flanking human immunodeficiency virus type 1 transframe protein p6* for spatiotemporal regulation of protease activation.

Authors:  Christine Ludwig; Andreas Leiherer; Ralf Wagner
Journal:  J Virol       Date:  2008-03-05       Impact factor: 5.103

9.  Processing sites in the human immunodeficiency virus type 1 (HIV-1) Gag-Pro-Pol precursor are cleaved by the viral protease at different rates.

Authors:  Steve C Pettit; Jeffrey N Lindquist; Andrew H Kaplan; Ronald Swanstrom
Journal:  Retrovirology       Date:  2005-11-01       Impact factor: 4.602

10.  Amino acid substitutions at the HIV-1 transframe region significantly impair virus infectivity.

Authors:  Fu-Hsien Yu; Kuo-Jung Huang; Chin-Tien Wang
Journal:  PLoS One       Date:  2022-01-27       Impact factor: 3.240
  10 in total

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