Literature DB >> 8793399

Detection of genes coding for extended-spectrum SHV beta-lactamases in clinical isolates by a molecular genetic method, and comparison with the E test.

M T Nüesch-Inderbinen1, H Hächler, F H Kayser.   

Abstract

A highly sensitive and specific method, termed PCR/NheI, for the detection of genes coding for SHV extended-spectrum beta-lactamases (ESBL) in clinical isolates is presented. It is based on polymerase chain reaction (PCR) amplification of the blaSHV genes, followed by restriction with NheI. Due to the glycine (positive 238) (SHV-non-ESBL)-->serine (position 238) (SHV-ESBL) mutation, only PCR fragments from the genes coding for SHV-ESBLs were cleaved. A commercially available test for ESBLs, the E test ESBL, identified 52% of our 29 clinical isolates carrying blaSHV-ESBL genes as ESBL producers.

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Year:  1996        PMID： 8793399     DOI： 10.1007/bf01690097

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN： 0934-9723            Impact factor:   3.267


  21 in total

1.  A standard numbering scheme for the class A beta-lactamases.

Authors:  R P Ambler; A F Coulson; J M Frère; J M Ghuysen; B Joris; M Forsman; R C Levesque; G Tiraby; S G Waley
Journal:  Biochem J       Date:  1991-05-15       Impact factor: 3.857

2.  Molecular cloning and nucleotide sequence of a new plasmid-coded Klebsiella pneumoniae beta-lactamase gene (SHV-2a) responsible for high-level cefotaxime resistance.

Authors:  A Podbielski; J Schönling; B Melzer; K Warnatz
Journal:  Zentralbl Bakteriol       Date:  1991-08

Review 3.  More extended-spectrum beta-lactamases.

Authors:  G A Jacoby; A A Medeiros
Journal:  Antimicrob Agents Chemother       Date:  1991-09       Impact factor: 5.191

Review 4.  Extended-spectrum beta-lactamases.

Authors:  A Philippon; R Labia; G Jacoby
Journal:  Antimicrob Agents Chemother       Date:  1989-08       Impact factor: 5.191

5.  Broad-spectrum, transmissible beta-lactamases.

Authors:  G A Jacoby; A A Medeiros; T F O'Brien; M E Pinto; H Jiang
Journal:  N Engl J Med       Date:  1988-09-15       Impact factor: 91.245

6.  Extended broad-spectrum beta-lactamases conferring transferable resistance to newer beta-lactam agents in Enterobacteriaceae: hospital prevalence and susceptibility patterns.

Authors:  V Jarlier; M H Nicolas; G Fournier; A Philippon
Journal:  Rev Infect Dis       Date:  1988 Jul-Aug

Review 7.  A functional classification scheme for beta-lactamases and its correlation with molecular structure.

Authors:  K Bush; G A Jacoby; A A Medeiros
Journal:  Antimicrob Agents Chemother       Date:  1995-06       Impact factor: 5.191

8.  Transferable resistance to third-generation cephalosporins in clinical isolates of Klebsiella pneumoniae: identification of CTX-1, a novel beta-lactamase.

Authors:  D Sirot; J Sirot; R Labia; A Morand; P Courvalin; A Darfeuille-Michaud; R Perroux; R Cluzel
Journal:  J Antimicrob Chemother       Date:  1987-09       Impact factor: 5.790

9.  Molecular characterization of the gene encoding SHV-3 beta-lactamase responsible for transferable cefotaxime resistance in clinical isolates of Klebsiella pneumoniae.

Authors:  M H Nicolas; V Jarlier; N Honore; A Philippon; S T Cole
Journal:  Antimicrob Agents Chemother       Date:  1989-12       Impact factor: 5.191

10.  Novel plasmid-mediated beta-lactamase (TEM-10) conferring selective resistance to ceftazidime and aztreonam in clinical isolates of Klebsiella pneumoniae.

Authors:  J P Quinn; D Miyashiro; D Sahm; R Flamm; K Bush
Journal:  Antimicrob Agents Chemother       Date:  1989-09       Impact factor: 5.191
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  74 in total

1.  Salmonella enterica serovar virchow with CTX-M-like beta-lactamase in Spain.

Authors:  E Simarro; F Navarro; J Ruiz; E Miró; J Gómez; B Mirelis
Journal:  J Clin Microbiol       Date:  2000-12       Impact factor: 5.948

Review 2.  Molecular detection of antimicrobial resistance.

Authors:  A C Fluit; M R Visser; F J Schmitz
Journal:  Clin Microbiol Rev       Date:  2001-10       Impact factor: 26.132

Review 3.  Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat.

Authors:  P A Bradford
Journal:  Clin Microbiol Rev       Date:  2001-10       Impact factor: 26.132

4.  Real-time PCR and melting curve analysis for reliable and rapid detection of SHV extended-spectrum beta-lactamases.

Authors:  C C Randegger; H Hächler
Journal:  Antimicrob Agents Chemother       Date:  2001-06       Impact factor: 5.191

5.  Expression of SHV-2 beta-lactamase and of reduced amounts of OmpK36 porin in Klebsiella pneumoniae results in increased resistance to cephalosporins and carbapenems.

Authors:  Brendan Crowley; Vicente J Benedí; Antonio Doménech-Sánchez
Journal:  Antimicrob Agents Chemother       Date:  2002-11       Impact factor: 5.191

Review 6.  Recent developments in beta lactamases and extended spectrum beta lactamases.

Authors:  Joumana N Samaha-Kfoury; George F Araj
Journal:  BMJ       Date:  2003-11-22

7.  Evaluation of the Etest ESBL and the BD Phoenix, VITEK 1, and VITEK 2 automated instruments for detection of extended-spectrum beta-lactamases in multiresistant Escherichia coli and Klebsiella spp.

Authors:  Maurine A Leverstein-van Hall; Ad C Fluit; Armand Paauw; Adrienne T A Box; Sylvain Brisse; Jan Verhoef
Journal:  J Clin Microbiol       Date:  2002-10       Impact factor: 5.948

8.  Epidemiological investigation of bloodstream infections by extended spectrum cephalosporin-resistant Escherichia coli in a Taiwanese teaching hospital.

Authors:  Jing-Jou Yan; Wen-Chien Ko; Jiunn-Jong Wu; Shu-Huei Tsai; Chin-Luan Chuang
Journal:  J Clin Microbiol       Date:  2004-07       Impact factor: 5.948

9.  Using nucleic acid microarrays to perform molecular epidemiology and detect novel β-lactamases: a snapshot of extended-spectrum β-lactamases throughout the world.

Authors:  Christine Lascols; Meredith Hackel; Andrea M Hujer; Steven H Marshall; Sam K Bouchillon; Daryl J Hoban; Stephen P Hawser; Robert E Badal; Robert A Bonomo
Journal:  J Clin Microbiol       Date:  2012-02-08       Impact factor: 5.948

10.  Post-surgical wound infections involving Enterobacteriaceae with reduced susceptibility to β-lactams in two Portuguese hospitals.

Authors:  Rúben Fernandes; Cristina Prudêncio
Journal:  Int Wound J       Date:  2010-08-16       Impact factor: 3.315
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