| Literature DB >> 8787781 |
M Imamura1, T Uemori, I Kato, Y Ishino.
Abstract
We identified a DNA polymerase with properties that differed from those of alpha-like DNA polymerase (Pol I), in Pyrococcus furiosus, a hyperthermophilic archaeon. The novel DNA polymerase (Pol II) was partially purified and characterized. The DNA polymerizing activity of Pol II was relatively sensitive to dideoxythymidine-triphosphate (ddTTP) and it was inhibited by N-ethylmaleimide, but not by aphidicolin. Activity staining gel electrophoresis showed that the DNA polymerizing activity was derived from a polypeptide with a molecular weight of 130000 on sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. The DNA elongation ability of Pol II using a natural DNA template was much lower than that of Pol I from this organism and DNA synthesis of Pol II seems to be non-processive.Entities:
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Year: 1995 PMID： 8787781 DOI： 10.1248/bpb.18.1647
Source DB: PubMed Journal: Biol Pharm Bull ISSN： 0918-6158 Impact factor: 2.233