Literature DB >> 8785716

Detection of Mycobacterium paratuberculosis in formalin-fixed paraffin-embedded tissues by the polymerase chain reaction.

Y Plante1, B W Remenda, B J Chelack, D M Haines.   

Abstract

The polymerase chain reaction (PCR) utilizing primers specific for the IS900 sequence of Mycobacterium paratuberculosis was applied to tissue sections of formalin-fixed, paraffin-embedded ileum from cattle with Johne's disease and the results compared to those obtained with acid-fast (Ziehl-Neelsen) and immunohistochemical staining. The PCR was positive in 19/21 tissues (90%) while Ziehl-Neelsen staining was positive in 18/21 (86%) and immunohistochemical staining in 21/21 (100%). The Ziehl-Neelsen and immunohistochemical stains are not, however, specific for M. paratuberculosis. The PCR for detection of M. paratuberculosis using the IS900 sequence is a specific and relatively sensitive method for confirmation of Johne's disease and its application to formalin-fixed, paraffin-embedded tissues may be useful for confirmation of dubious cases, for retrospective studies and for epidemiological analyses.

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Year:  1996        PMID： 8785716      PMCID： PMC1263816     

Source DB:  PubMed          Journal:  Can J Vet Res        ISSN： 0830-9000            Impact factor:   1.310


  25 in total

Review 1.  Technical considerations for developing enzyme immunohistochemical staining procedures on formalin-fixed paraffin-embedded tissues for diagnostic pathology.

Authors:  D M Haines; B J Chelack
Journal:  J Vet Diagn Invest       Date:  1991-01       Impact factor: 1.279

2.  Application of a polymerase chain reaction for the detection of Mycobacterium leprae in skin tissues.

Authors:  M Y de Wit; W R Faber; S R Krieg; J T Douglas; S B Lucas; N Montreewasuwat; S R Pattyn; R Hussain; J M Ponnighaus; R A Hartskeerl
Journal:  J Clin Microbiol       Date:  1991-05       Impact factor: 5.948

3.  Comparison of a commercial DNA probe test and three cultivation procedures for detection of Mycobacterium paratuberculosis in bovine feces.

Authors:  D L Whipple; P A Kapke; P R Andersen
Journal:  J Vet Diagn Invest       Date:  1992-01       Impact factor: 1.279

4.  Polyclonal antibodies raised against Bacillus Calmette-Guerin, Mycobacterium duvalii, and Mycobacterium paratuberculosis used to detect mycobacteria in tissue with the use of immunohistochemical techniques.

Authors:  E L Wiley; T J Mulhollan; B Beck; J A Tyndall; R G Freeman
Journal:  Am J Clin Pathol       Date:  1990-09       Impact factor: 2.493

5.  Diagnosis of tuberculosis by DNA amplification in clinical practice evaluation.

Authors:  A Brisson-Noel; C Aznar; C Chureau; S Nguyen; C Pierre; M Bartoli; R Bonete; G Pialoux; B Gicquel; G Garrigue
Journal:  Lancet       Date:  1991-08-10       Impact factor: 79.321

6.  Identification of two groups of Mycobacterium paratuberculosis strains by restriction endonuclease analysis and DNA hybridization.

Authors:  D M Collins; D M Gabric; G W de Lisle
Journal:  J Clin Microbiol       Date:  1990-07       Impact factor: 5.948

7.  A DNA probe for the detection of Mycobacterium paratuberculosis.

Authors:  R E Ambrosio; Y Harris; H F Huchzermeyer
Journal:  Vet Microbiol       Date:  1991-01       Impact factor: 3.293

8.  Use of highly specific DNA probes and the polymerase chain reaction to detect Mycobacterium paratuberculosis in Johne's disease.

Authors:  P H Vary; P R Andersen; E Green; J Hermon-Taylor; J J McFadden
Journal:  J Clin Microbiol       Date:  1990-05       Impact factor: 5.948

9.  Amplification of 16S rRNA sequences to detect Mycobacterium paratuberculosis.

Authors:  J W van der Giessen; A Eger; J Haagsma; R M Haring; W Gaastra; B A van der Zeijst
Journal:  J Med Microbiol       Date:  1992-04       Impact factor: 2.472

10.  Specific detection of Mycobacterium paratuberculosis by DNA hybridisation with a fragment of the insertion element IS900.

Authors:  M T Moss; E P Green; M L Tizard; Z P Malik; J Hermon-Taylor
Journal:  Gut       Date:  1991-04       Impact factor: 23.059
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  2 in total

1.  Duplex PCR for differential identification of Mycobacterium bovis, M. avium, and M. avium subsp. paratuberculosis in formalin- fixed paraffin-embedded tissues from cattle.

Authors:  C Coetsier; P Vannuffel; N Blondeel; J F Denef; C Cocito; J L Gala
Journal:  J Clin Microbiol       Date:  2000-08       Impact factor: 5.948

2.  Detection of Mycobacterium avium subsp. paratuberculosis in infected tissues by new species-specific immunohistological procedures.

Authors:  C Coetsier; X Havaux; F Mattelard; S Sadatte; F Cormont; K Buergelt; B Limbourg; D Latinne; H Bazin; J F Denef; C Cocito
Journal:  Clin Diagn Lab Immunol       Date:  1998-07
  2 in total

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