| Literature DB >> 2024441 |
R E Ambrosio1, Y Harris, H F Huchzermeyer.
Abstract
A genomic library of DNA extracted from Mycobacterium paratuberculosis was constructed in the expression vector lambda gt 11. The library was screened by plaque hybridization with labelled M. paratuberculosis genomic DNA as probe. Strongly hybridizing plaques were isolated and their DNA extracted and characterised for M. paratuberculosis specificity by hybridization to DNA from other Mycobacteriaceae. A clone was obtained which was specific for M. paratuberculosis. DNA from this clone could detect 7 ng M. paratuberculosis DNA.Entities:
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Year: 1991 PMID： 2024441 DOI： 10.1016/0378-1135(91)90044-g
Source DB: PubMed Journal: Vet Microbiol ISSN： 0378-1135 Impact factor: 3.293