Literature DB >> 8784603

Comparison of amplicor and 32-kilodalton PCR for detection of Mycobacterium tuberculosis from sputum specimens.

H Soini1, S A Agha, A El-Fiky, M K Viljanen.   

Abstract

The commercial PCR test Amplicor was compared with the 32-kDa PCR for detection of Mycobacterium tuberculosis from 76 sputum specimens from Egyptian patients. Both tests performed with rather equal efficacy (resolved sensitivity of 88.9% for both tests; specificity of 98.0% for Amplicor and 93.9% for 32-kDa PCR). PCR was found to be useful in detection of auramine fluorescent stain-positive, culture-negative specimens.

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Year:  1996        PMID: 8784603      PMCID: PMC229128     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  15 in total

1.  Identification of mycobacteria by PCR-based sequence determination of the 32-kilodalton protein gene.

Authors:  H Soini; E C Böttger; M K Viljanen
Journal:  J Clin Microbiol       Date:  1994-12       Impact factor: 5.948

2.  Sensitivity and specificity of PCR for detection of Mycobacterium tuberculosis: a blind comparison study among seven laboratories.

Authors:  G T Noordhoek; A H Kolk; G Bjune; D Catty; J W Dale; P E Fine; P Godfrey-Faussett; S N Cho; T Shinnick; S B Svenson
Journal:  J Clin Microbiol       Date:  1994-02       Impact factor: 5.948

3.  Diagnosis of tuberculosis by DNA amplification in clinical practice evaluation.

Authors:  A Brisson-Noel; C Aznar; C Chureau; S Nguyen; C Pierre; M Bartoli; R Bonete; G Pialoux; B Gicquel; G Garrigue
Journal:  Lancet       Date:  1991-08-10       Impact factor: 79.321

4.  Detection of Mycobacterium tuberculosis in clinical samples by using polymerase chain reaction and a nonradioactive detection system.

Authors:  A H Kolk; A R Schuitema; S Kuijper; J van Leeuwen; P W Hermans; J D van Embden; R A Hartskeerl
Journal:  J Clin Microbiol       Date:  1992-10       Impact factor: 5.948

5.  Direct detection of Mycobacterium tuberculosis in respiratory specimens in a clinical laboratory by polymerase chain reaction.

Authors:  B A Forbes; K E Hicks
Journal:  J Clin Microbiol       Date:  1993-07       Impact factor: 5.948

6.  Direct detection of Mycobacterium tuberculosis in sputum by polymerase chain reaction and DNA hybridization.

Authors:  F S Nolte; B Metchock; J E McGowan; A Edwards; O Okwumabua; C Thurmond; P S Mitchell; B Plikaytis; T Shinnick
Journal:  J Clin Microbiol       Date:  1993-07       Impact factor: 5.948

7.  Large-scale use of polymerase chain reaction for detection of Mycobacterium tuberculosis in a routine mycobacteriology laboratory.

Authors:  J E Clarridge; R M Shawar; T M Shinnick; B B Plikaytis
Journal:  J Clin Microbiol       Date:  1993-08       Impact factor: 5.948

8.  Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by Amplicor PCR.

Authors:  D F Moore; J I Curry
Journal:  J Clin Microbiol       Date:  1995-10       Impact factor: 5.948

Review 9.  Tuberculosis: commentary on a reemergent killer.

Authors:  B R Bloom; C J Murray
Journal:  Science       Date:  1992-08-21       Impact factor: 47.728

10.  Clinical evaluation of a Mycobacterium tuberculosis PCR assay.

Authors:  J Beige; J Lokies; T Schaberg; U Finckh; M Fischer; H Mauch; H Lode; B Köhler; A Rolfs
Journal:  J Clin Microbiol       Date:  1995-01       Impact factor: 5.948

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  5 in total

Review 1.  Current evidence on diagnostic accuracy of commercially based nucleic acid amplification tests for the diagnosis of pulmonary tuberculosis.

Authors:  S Greco; E Girardi; A Navarra; C Saltini
Journal:  Thorax       Date:  2006-05-31       Impact factor: 9.139

2.  Diagnostic accuracy of in-house PCR for pulmonary tuberculosis in smear-positive patients: meta-analysis and metaregression.

Authors:  S Greco; M Rulli; E Girardi; C Piersimoni; C Saltini
Journal:  J Clin Microbiol       Date:  2009-01-14       Impact factor: 5.948

3.  Cost-effectiveness analysis of PCR for the rapid diagnosis of pulmonary tuberculosis.

Authors:  Luciene C Scherer; Rosa D Sperhacke; Antonio Ruffino-Netto; Maria Lr Rossetti; Claudia Vater; Paul Klatser; Afrânio L Kritski
Journal:  BMC Infect Dis       Date:  2009-12-31       Impact factor: 3.090

4.  In-house nucleic acid amplification tests for the detection of Mycobacterium tuberculosis in sputum specimens: meta-analysis and meta-regression.

Authors:  Laura L Flores; Madhukar Pai; John M Colford; Lee W Riley
Journal:  BMC Microbiol       Date:  2005-10-03       Impact factor: 3.605

5.  Commercial nucleic-acid amplification tests for diagnosis of pulmonary tuberculosis in respiratory specimens: meta-analysis and meta-regression.

Authors:  Daphne I Ling; Laura L Flores; Lee W Riley; Madhukar Pai
Journal:  PLoS One       Date:  2008-02-06       Impact factor: 3.240

  5 in total

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