Literature DB >> 8781966

A fluorometric assay of ceramide glycanase with 4-methylumbelliferyl beta-D-lactoside derivatives.

L X Wang1, N V Pavlova, S C Li, Y T Li, Y C Lee.   

Abstract

4-Methylumbelliferyl 6'-O-benzyl-beta-D-lactoside (6'Bn-MU-Lac) and some related compounds were synthesized via different selective reactions including phase-transfer glycosylation. Their suitability as substrates for a fluorometric assay of ceramide glycanase (CGase) was evaluated. Among others, the 6'Bn-MU-Lac, which is resistant to exogalactosidase, was found to be a suitable substrate for routine assay of the CGase activity. For American leech CGase, the K(m) value is 0.232 mM at pH 5.

Entities:  

Mesh：

Substances：

Year:  1996        PMID： 8781966     DOI： 10.1007/bf00731468

Source DB:  PubMed          Journal:  Glycoconj J        ISSN： 0282-0080            Impact factor:   2.916


  9 in total

1.  Purification and characterization of membrane-bound endoglycoceramidase from Corynebacterium sp.

Authors:  H Ashida; K Yamamoto; H Kumagai; T Tochikura
Journal:  Eur J Biochem       Date:  1992-04-15

2.  Ceramide glycanase from leech, Hirudo medicinalis, and earthworm, Lumbricus terrestris.

Authors:  Y T Li; S C Li
Journal:  Methods Enzymol       Date:  1989       Impact factor: 1.600

3.  Ceramide glycanase from the earthworm, Lumbricus terrestris.

Authors:  B Z Carter; S C Li; Y T Li
Journal:  Biochem J       Date:  1992-07-15       Impact factor: 3.857

4.  Synthesis of neoglycoconjugates using oligosaccharide transferring activity of ceramide glycanase.

Authors:  Y T Li; B Z Carter; B N Rao; H Schweingruber; S C Li
Journal:  J Biol Chem       Date:  1991-06-15       Impact factor: 5.157

5.  Occurrence of ceramide-glycanase in the earthworm, Lumbricus terrestris.

Authors:  Y T Li; Y Ishikawa; S C Li
Journal:  Biochem Biophys Res Commun       Date:  1987-11-30       Impact factor: 3.575

6.  A unique glycosphingolipid-splitting enzyme (ceramide-glycanase from leech) cleaves the linkage between the oligosaccharide and the ceramide.

Authors:  S C Li; R DeGasperi; J E Muldrey; Y T Li
Journal:  Biochem Biophys Res Commun       Date:  1986-11-26       Impact factor: 3.575

7.  Initial rates. A new plot.

Authors:  E A Boeker
Journal:  Biochem J       Date:  1982-04-01       Impact factor: 3.857

8.  A novel glycosphingolipid-degrading enzyme cleaves the linkage between the oligosaccharide and ceramide of neutral and acidic glycosphingolipids.

Authors:  M Ito; T Yamagata
Journal:  J Biol Chem       Date:  1986-10-25       Impact factor: 5.157

9.  Isolation and characterization of ceramide glycanase from the leech, Macrobdella decora.

Authors:  B Zhou; S C Li; R A Laine; R T Huang; Y T Li
Journal:  J Biol Chem       Date:  1989-07-25       Impact factor: 5.157
  9 in total
  1 in total

1.  Teaming up synthetic chemistry and histochemistry for activity screening in galectin-directed inhibitor design.

Authors:  René Roy; Yihong Cao; Herbert Kaltner; Naresh Kottari; Tze Chieh Shiao; Karima Belkhadem; Sabine André; Joachim C Manning; Paul V Murphy; Hans-Joachim Gabius
Journal:  Histochem Cell Biol       Date:  2016-12-24       Impact factor: 4.304
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.