Literature DB >> 8781885

Detection of vertical transmission of human immunodeficiency virus type 1 by a commercial polymerase chain reaction assay.

P Bermúdez1, P Moreno, R Delgado, J T Ramos, J Ruiz-Contreras, A R Noriega.   

Abstract

In a prospective study, a commercial polymerase chain reaction (PCR) system was compared with a conventional procedure, based on PCR and hybridization with a radio-labeled probe, for the detection of human immunodeficiency virus (HIV) infection in 131 blood samples from 80 children born to HIV-seropositive mothers. Twenty-three of these children were HIV infected. The sensitivity and specificity of the commercial assay as compared with the conventional PCR procedure were 100% and 95.1%, respectively. This commercial method simplifies the performance of the conventional PCR technique and can be used to detect HIV type 1 vertical transmission.

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Year:  1996        PMID： 8781885     DOI： 10.1007/bf01695666

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN： 0934-9723            Impact factor:   3.267


  11 in total

1.  Comparison of three nonradioisotopic polymerase chain reaction-based methods for detection of human immunodeficiency virus type 1.

Authors:  A J Whetsell; J B Drew; G Milman; R Hoff; E A Dragon; K Adler; J Hui; P Otto; P Gupta; H Farzadegan
Journal:  J Clin Microbiol       Date:  1992-04       Impact factor: 5.948

2.  Antigen detection, virus culture, polymerase chain reaction, and in vitro antibody production in the diagnosis of vertically transmitted HIV-1 infection.

Authors:  A De Rossi; A E Ades; F Mammano; A Del Mistro; A Amadori; C Giaquinto; L Chieco-Bianchi
Journal:  AIDS       Date:  1991-01       Impact factor: 4.177

Review 3.  Use of virologic assays for detection of human immunodeficiency virus in clinical trials: recommendations of the AIDS Clinical Trials Group Virology Committee.

Authors:  S Hammer; C Crumpacker; R D'Aquila; B Jackson; J Lathey; D Livnat; P Reichelderfer
Journal:  J Clin Microbiol       Date:  1993-10       Impact factor: 5.948

4.  Acid dissociation of immune complexes improves diagnostic utility of p24 antigen detection in perinatally acquired human immunodeficiency virus infection.

Authors:  T C Quinn; R Kline; M W Moss; R A Livingston; N Hutton
Journal:  J Infect Dis       Date:  1993-05       Impact factor: 5.226

5.  Establishment of a quality assurance program for human immunodeficiency virus type 1 DNA polymerase chain reaction assays by the AIDS Clinical Trials Group. ACTG PCR Working Group, and the ACTG PCR Virology Laboratories.

Authors:  J B Jackson; J Drew; H J Lin; P Otto; J W Bremer; F B Hollinger; S M Wolinsky
Journal:  J Clin Microbiol       Date:  1993-12       Impact factor: 5.948

Review 6.  Report of a consensus workshop, Siena, Italy, January 17-18, 1992. Early diagnosis of HIV infection in infants.

Authors: 
Journal:  J Acquir Immune Defic Syndr (1988)       Date:  1992

7.  Rapid screening for early detection of mother-to-child transmission of human immunodeficiency virus type 1.

Authors:  S Cassol; A Butcher; S Kinard; J Spadoro; T Sy; N Lapointe; S Read; P Gomez; M Fauvel; C Major
Journal:  J Clin Microbiol       Date:  1994-11       Impact factor: 5.948

8.  Risk factors for mother-to-child transmission of HIV-1.

Authors: 
Journal:  Lancet       Date:  1992-04-25       Impact factor: 79.321

9.  Detection of human immunodeficiency virus type 1 infection in young pediatric patients by using polymerase chain reaction and biotinylated probes.

Authors:  C D Brandt; T A Rakusan; A V Sison; S H Josephs; E S Saxena; K D Herzog; R H Parrott; J L Sever
Journal:  J Clin Microbiol       Date:  1992-01       Impact factor: 5.948

10.  Biological and biochemical characterization of a cloned Leu-3- cell surviving infection with the acquired immune deficiency syndrome retrovirus.

Authors:  T M Folks; D Powell; M Lightfoote; S Koenig; A S Fauci; S Benn; A Rabson; D Daugherty; H E Gendelman; M D Hoggan
Journal:  J Exp Med       Date:  1986-07-01       Impact factor: 14.307
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