Literature DB >> 8769306

DNA-nuclease activity of the single-chain ribosome-inactivating proteins dianthin 30, saporin 6 and gelonin.

L Roncuzzi1, A Gasperi-Campani.   

Abstract

The single-chain ribosome-inactivating proteins (scRIPs) from plant origin are antiviral and antiproliferative agents employed in the preparation of immunotoxins. Similarly to the A-chains of ricin, sc-RIPs act as rRNA N-glycosidases. We demonstrate here that dianthin 30, saporin 6 and gelonin exert a specific nuclease activity on supercoiled DNA. Four specific sites of cleavage introduced by dianthin 30 and by saporin 6 and two specific sites of cleavage introduced by gelonin have been identified and mapped in pBR322.

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Year:  1996        PMID： 8769306     DOI： 10.1016/0014-5793(96)00776-4

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  19 in total

1.  An additional mechanism of ribosome-inactivating protein cytotoxicity: degradation of extrachromosomal DNA.

Authors:  E Nicolas; I D Goodyer; T F Taraschi
Journal:  Biochem J       Date:  1997-10-15       Impact factor: 3.857

2.  Polynucleotide:adenosine glycosidase activity of ribosome-inactivating proteins: effect on DNA, RNA and poly(A).

Authors:  L Barbieri; P Valbonesi; E Bonora; P Gorini; A Bolognesi; F Stirpe
Journal:  Nucleic Acids Res       Date:  1997-02-01       Impact factor: 16.971

3.  Biological activities of the antiviral protein BE27 from sugar beet (Beta vulgaris L.).

Authors:  Rosario Iglesias; Lucía Citores; Antimo Di Maro; José M Ferreras
Journal:  Planta       Date:  2014-10-19       Impact factor: 4.116

4.  Functional Analysis of a Type-I Ribosome Inactivating Protein Balsamin from Momordica balsamina with Anti-Microbial and DNase Activity.

Authors:  Parminder Kaur Ajji; Ken Walder; Munish Puri
Journal:  Plant Foods Hum Nutr       Date:  2016-09       Impact factor: 3.921

5.  The differential catalytic activity of ribosome-inactivating proteins saporin 5 and 6 is due to a single substitution at position 162.

Authors:  Paroma Ghosh; Janendra K Batra
Journal:  Biochem J       Date:  2006-11-15       Impact factor: 3.857

6.  Characterization of two novel type I ribosome-inactivating proteins from the storage roots of the andean crop Mirabilis expansa.

Authors:  J M Vivanco; B J Savary; H E Flores
Journal:  Plant Physiol       Date:  1999-04       Impact factor: 8.340

7.  Isolation and characterization of a novel ribosome-inactivating protein from root cultures of pokeweed and its mechanism of secretion from roots.

Authors:  Sang-Wook Park; Christopher B Lawrence; James C Linden; Jorge M Vivanco
Journal:  Plant Physiol       Date:  2002-09       Impact factor: 8.340

8.  Isolation and characterization of an RIP (ribosome-inactivating protein)-like protein from tobacco with dual enzymatic activity.

Authors:  Neelam Sharma; Sang-Wook Park; Ramarao Vepachedu; Luigi Barbieri; Marialibera Ciani; Fiorenzo Stirpe; Brett J Savary; Jorge M Vivanco
Journal:  Plant Physiol       Date:  2003-12-11       Impact factor: 8.340

9.  Both N- and C-terminal regions are essential for cinnamomin A-chain to deadenylate ribosomal RNA and supercoiled double-stranded DNA.

Authors:  Wen-Jun He; Wang-Yi Liu
Journal:  Biochem J       Date:  2004-01-01       Impact factor: 3.857

10.  Insights into the mechanism of cell death induced by saporin delivered into cancer cells by an antibody fusion protein targeting the transferrin receptor 1.

Authors:  Tracy R Daniels-Wells; Gustavo Helguera; José A Rodríguez; Lai Sum Leoh; Michael A Erb; Graciel Diamante; David Casero; Matteo Pellegrini; Otoniel Martínez-Maza; Manuel L Penichet
Journal:  Toxicol In Vitro       Date:  2012-10-17       Impact factor: 3.500
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