Literature DB >> 8766820

Kinetic and spectral properties of pea cytosolic ascorbate peroxidase.

L A Marquez1, M Quitoriano, B A Zilinskas, H B Dunford.   

Abstract

Sufficient highly purified native pea cytosolic ascorbate peroxidase was obtained to characterize some of its kinetic and spectral properties. Its rate constant for compound I formation from reaction with H2O2 is 4.O x 10(7) M-1 s-1, somewhat faster than is typical for peroxidases. Compound I has the typical optical spectrum of an iron(IV)-porphyrin-pi-cation radical, despite considerable homology with yeast cytochrome c peroxidase. The rate constant for compound I reduction by ascorbate is extremely fast (8.0 x 10(7) M-1 S-1 at pH 7.8), again in marked contrast to the behavior of the yeast enzyme. The pH-rate profile for compound I formation indicates a pKa value of 5.0 for a group affecting the active site reaction.

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Year:  1996        PMID： 8766820     DOI： 10.1016/0014-5793(96)00562-5

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  11 in total

1.  Leishmania major encodes an unusual peroxidase that is a close homologue of plant ascorbate peroxidase: a novel role of the transmembrane domain.

Authors:  Subrata Adak; Alok K Datta
Journal:  Biochem J       Date:  2005-09-01       Impact factor: 3.857

2.  Purification and characterization of a novel class III peroxidase isoenzyme from tea leaves.

Authors:  M Kvaratskhelia; C Winkel; R N Thorneley
Journal:  Plant Physiol       Date:  1997-08       Impact factor: 8.340

Review 3.  Heme enzyme structure and function.

Authors:  Thomas L Poulos
Journal:  Chem Rev       Date:  2014-01-08       Impact factor: 60.622

4.  Reactivity of deoxy- and oxyferrous dehaloperoxidase B from Amphitrite ornata: identification of compound II and its ferrous-hydroperoxide precursor.

Authors:  Jennifer D'Antonio; Reza A Ghiladi
Journal:  Biochemistry       Date:  2011-06-15       Impact factor: 3.162

5.  Spectroscopic and mechanistic investigations of dehaloperoxidase B from Amphitrite ornata.

Authors:  Jennifer D'Antonio; Edward L D'Antonio; Matthew K Thompson; Edmond F Bowden; Stefan Franzen; Tatyana Smirnova; Reza A Ghiladi
Journal:  Biochemistry       Date:  2010-08-10       Impact factor: 3.162

Review 6.  Thirty years of heme peroxidase structural biology.

Authors:  Thomas L Poulos
Journal:  Arch Biochem Biophys       Date:  2010-03-03       Impact factor: 4.013

7.  Kinetic study of the inactivation of ascorbate peroxidase by hydrogen peroxide.

Authors:  A N Hiner; J N Rodríguez-López; M B Arnao; E Lloyd Raven; F García-Cánovas; M Acosta
Journal:  Biochem J       Date:  2000-06-01       Impact factor: 3.857

8.  The role of quaternary interactions on the stability and activity of ascorbate peroxidase.

Authors:  D Mandelman; F P Schwarz; H Li; T L Poulos
Journal:  Protein Sci       Date:  1998-10       Impact factor: 6.725

9.  Trypanosoma cruzi expresses a plant-like ascorbate-dependent hemoperoxidase localized to the endoplasmic reticulum.

Authors:  Shane R Wilkinson; Samson O Obado; Isabel L Mauricio; John M Kelly
Journal:  Proc Natl Acad Sci U S A       Date:  2002-09-26       Impact factor: 11.205

10.  Development of a Genome-Edited Tomato With High Ascorbate Content During Later Stage of Fruit Ripening Through Mutation of SlAPX4.

Authors:  Ju Hui Do; Seo Young Park; Se Hee Park; Hyun Min Kim; Sang Hoon Ma; Thanh Dat Mai; Jae Sung Shim; Young Hee Joung
Journal:  Front Plant Sci       Date:  2022-04-12       Impact factor: 5.753
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