OBJECTIVE: The human genes MAGE-1 and -3 encode tumor-specific peptide antigens, which are recognized by autologous cytotoxic T lymphocytes. The antigens coded by those genes may be useful for cancer immunotherapy. There is, however, little information on the expression of these genes in human colorectal carcinomas. METHOD: The expression of MAGE-1, -2, and -3 genes in 54 pairs of tumor and corresponding normal tissue specimens of the colorectum was determined by means of reverse transcription polymerase chain reaction. The induction of MAGE-1, -2, -3, and -4 gene expression in eight colorectal carcinoma cell lines also was examined by use of a demethylating agent, 5-Aza-2'-deoxycytidine (DAC). RESULTS: The expression of MAGE genes was not recognized in normal colorectal tissues at all. In tumor tissue specimens, the expression of MAGE-1, -2, and -3 was recognized in 16 (30%), 15 (28%), and 11 (20%) patients, respectively. The expression was seen frequently in patients with liver metastasis (p < 0.01). Although MAGE-1 or -3 genes were not induced by DAC, MAGE-2 or -4 genes were induced in three of four MAGE-2 negative cell lines or three of seven MAGE-4 negative cell lines, respectively. CONCLUSIONS: The MAGE genes were expressed exclusively in tumor tissues of one third of patients with colorectal carcinoma. The identification of such tumor rejection antigens is considered to uncover a new possibility for the specific immunotherapy of colorectal carcinoma. The demethylating agent may increase the number of patients who might be candidates for MAGE-specific immunotherapy.
OBJECTIVE: The human genes MAGE-1 and -3 encode tumor-specific peptide antigens, which are recognized by autologous cytotoxic T lymphocytes. The antigens coded by those genes may be useful for cancer immunotherapy. There is, however, little information on the expression of these genes in humancolorectal carcinomas. METHOD: The expression of MAGE-1, -2, and -3 genes in 54 pairs of tumor and corresponding normal tissue specimens of the colorectum was determined by means of reverse transcription polymerase chain reaction. The induction of MAGE-1, -2, -3, and -4 gene expression in eight colorectal carcinoma cell lines also was examined by use of a demethylating agent, 5-Aza-2'-deoxycytidine (DAC). RESULTS: The expression of MAGE genes was not recognized in normal colorectal tissues at all. In tumor tissue specimens, the expression of MAGE-1, -2, and -3 was recognized in 16 (30%), 15 (28%), and 11 (20%) patients, respectively. The expression was seen frequently in patients with liver metastasis (p < 0.01). Although MAGE-1 or -3 genes were not induced by DAC, MAGE-2 or -4 genes were induced in three of four MAGE-2 negative cell lines or three of seven MAGE-4 negative cell lines, respectively. CONCLUSIONS: The MAGE genes were expressed exclusively in tumor tissues of one third of patients with colorectal carcinoma. The identification of such tumor rejection antigens is considered to uncover a new possibility for the specific immunotherapy of colorectal carcinoma. The demethylating agent may increase the number of patients who might be candidates for MAGE-specific immunotherapy.
Authors: H Inoue; J Li; M Honda; H Nakashima; K Shibuta; S Arinaga; H Ueo; M Mori; T Akiyoshi Journal: Int J Cancer Date: 1995-02-20 Impact factor: 7.396
Authors: P van der Bruggen; J Bastin; T Gajewski; P G Coulie; P Boël; C De Smet; C Traversari; A Townsend; T Boon Journal: Eur J Immunol Date: 1994-12 Impact factor: 5.532
Authors: H Inoue; M Mori; J Li; K Mimori; M Honda; H Nakashima; K Mafune; Y Tanaka; T Akiyoshi Journal: Int J Cancer Date: 1995-11-15 Impact factor: 7.396
Authors: P van der Bruggen; C Traversari; P Chomez; C Lurquin; E De Plaen; B Van den Eynde; A Knuth; T Boon Journal: Science Date: 1991-12-13 Impact factor: 47.728
Authors: C Traversari; P van der Bruggen; B Van den Eynde; P Hainaut; C Lemoine; N Ohta; L Old; T Boon Journal: Immunogenetics Date: 1992 Impact factor: 2.846
Authors: G F Hofbauer; C Schaefer; C Noppen; R Böni; J Kamarashev; F O Nestle; G C Spagnoli; R Dummer Journal: Am J Pathol Date: 1997-12 Impact factor: 4.307
Authors: A Miyamoto; Y Fujiwara; M Sakon; H Nagano; Y Sugita; M Kondo; H Eguchi; K Dono; K Umeshita; S Nakamori; M Monden Journal: Dig Dis Sci Date: 2000-07 Impact factor: 3.199
Authors: K Kariyama; T Higashi; Y Kobayashi; K Nouso; H Nakatsukasa; T Yamano; M Ishizaki; T Kaneyoshi; N Toshikuni; T Ohnishi; K Fujiwara; E Nakayama; L Terracciano; G C Spagnoli; T Tsuji Journal: Br J Cancer Date: 1999-11 Impact factor: 7.640