| Literature DB >> 8751388 |
M Meddeb1, A Valent, G Danglot, V C Nguyen, A Duverger, F Fouquet, M J Terrier-Lacombe, O Oberlin, A Bernheim.
Abstract
This report describes a case of rhabdomyosarcoma associated with a 2;13 translocation and multiple double minute chromosomes. The origin of the amplified DNA was identified using comparative genomic hybridization, which pinpointed a unique spot at 12q13-->q14. Band 12q13 has been shown to contain several genes that are occasionally amplified in other sarcomas. Fluorescene in situ hybridization to tumor metaphases with probes specific for this region indicated that the double minutes contained the MDM2 gene but not the CDK4 gene. MDM2 amplification was further quantified by Southern hybridization, which showed a mean value of 25 copies per haploid genome. This is the first example of MDM2 amplification in a rhabdomyosarcoma.Entities:
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Year: 1996 PMID： 8751388 DOI： 10.1159/000134368
Source DB: PubMed Journal: Cytogenet Cell Genet ISSN： 0301-0171