PURPOSE: This study was performed to determine whether frozen-thawed mouse round spermatids can fertilize oocytes and contribute to normal embryo development. METHODS: Freshly collected mouse testicular cells were frozen in PBS containing 7.5% glycerol and 7.5% fetal bovine serum. After thawing and removal of the cryoprotectants, round spermatids were selected and injected individually into mature oocytes which had been previously activated with Sr(2+)-containing Ca(2+)-free medium. RESULTS: After thawing, 75-85% of testicular cells were alive. About 90% of the oocytes were fertilized by intracytoplasmic injection of frozen-thawed round spermatids; 11% (17/150) of embryos transferred to foster mothers developed into normal offspring. CONCLUSIONS: Mouse round spermatids can be cryopreserved for production of normal offspring.
PURPOSE: This study was performed to determine whether frozen-thawed mouse round spermatids can fertilize oocytes and contribute to normal embryo development. METHODS: Freshly collected mouse testicular cells were frozen in PBS containing 7.5% glycerol and 7.5% fetal bovine serum. After thawing and removal of the cryoprotectants, round spermatids were selected and injected individually into mature oocytes which had been previously activated with Sr(2+)-containing Ca(2+)-free medium. RESULTS: After thawing, 75-85% of testicular cells were alive. About 90% of the oocytes were fertilized by intracytoplasmic injection of frozen-thawed round spermatids; 11% (17/150) of embryos transferred to foster mothers developed into normal offspring. CONCLUSIONS:Mouse round spermatids can be cryopreserved for production of normal offspring.
Authors: N V Sofikitis; I Miyagawa; E Agapitos; P Pasyianos; T Toda; W J Hellstrom; H Kawamura Journal: J Assist Reprod Genet Date: 1994-08 Impact factor: 3.412
Authors: Alyssa N Kruger; Michele A Brogley; Jamie L Huizinga; Jeffrey M Kidd; Dirk G de Rooij; Yueh-Chiang Hu; Jacob L Mueller Journal: Curr Biol Date: 2019-10-17 Impact factor: 10.834