| Literature DB >> 16945902 |
Takashi Shinohara1, Megumi Kato, Masanori Takehashi, Jiyoung Lee, Shinichiro Chuma, Norio Nakatsuji, Mito Kanatsu-Shinohara, Masumi Hirabayashi.
Abstract
Spermatogonial transplantation has demonstrated a unique opportunity for studying spermatogenesis and provided an assay for spermatogonial stem cells. However, it has remained unknown whether germ cells that matured in a xenogeneic environment are functionally normal. In this investigation, we demonstrate the successful production of xenogeneic offspring by using spermatogonial transplantation. Rat spermatogonial stem cells were collected from immature testis and transplanted into the seminiferous tubules of busulfan-treated nude mouse testis. Using rat spermatids or spermatozoa that developed in xenogeneic surrogate mice, rat offspring were born from fresh and cryopreserved donor cells after microinsemination with rat oocytes. These offspring were fertile and had a normal imprinting pattern. The xenogeneic offspring production by interspecies germ cell transplantation and in vitro microinsemination will become a powerful tool in animal transgenesis and species conservation.Entities:
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Year: 2006 PMID： 16945902 PMCID： PMC1564227 DOI： 10.1073/pnas.0604205103
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205