Literature DB >> 8735126

Enhanced amplification of hepatitis C virus (HCV) cDNA by PCR: detection of HCV RNA in archival sera.

A M Beardsley1, E J Gowans, C J Burrell, B P Marmion.   

Abstract

A reverse transcription-PCR assay which successfully amplified hepatitis C virus RNA from poorly stored archival sera was optimized. Maximum sensitivity was achieved with Moloney murine leukemia virus RNase H- reverse transcriptase and by a single round of PCR amplification of a short (112-bp) fragment of the 5' untranslated region of the viral genome.

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Year:  1996        PMID： 8735126      PMCID： PMC229070          DOI： 10.1128/jcm.34.6.1581-1582.1996

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  13 in total

1.  Improvement of HCV genome detection with "short" PCR products.

Authors:  J A Garson; C J Ring; P W Tuke
Journal:  Lancet       Date:  1991-12-07       Impact factor: 79.321

2.  Chronic liver disease in asymptomatic hepatitis C antibody positive blood donors.

Authors:  R P Hardiman; D R Shaw; J T La Brooy; L Rozen; W B de Boer; R Rowland; A M Beardsley; E J Gowans
Journal:  Aust N Z J Med       Date:  1993-04

3.  First-strand cDNA synthesis primed with oligo(dT).

Authors:  M S Krug; S L Berger
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

4.  Avoiding false positives with PCR.

Authors:  S Kwok; R Higuchi
Journal:  Nature       Date:  1989-05-18       Impact factor: 49.962

5.  Isolation of hepatitis C virus RNA from serum for reverse transcription-PCR.

Authors:  F S Nolte; C Thurmond; P S Mitchell
Journal:  J Clin Microbiol       Date:  1994-02       Impact factor: 5.948

6.  A comparison of hepatitis C virus (HCV)-RNA and--antibody as markers of infection and predictors of infectivity.

Authors:  A M Beardsley; J T LaBrooy; L Rozen; E J Gowans
Journal:  Aust N Z J Med       Date:  1994-04

7.  Secondary structure of the 5' nontranslated regions of hepatitis C virus and pestivirus genomic RNAs.

Authors:  E A Brown; H Zhang; L H Ping; S M Lemon
Journal:  Nucleic Acids Res       Date:  1992-10-11       Impact factor: 16.971

8.  Impact of specimen handling and storage on detection of hepatitis C virus RNA.

Authors:  M P Busch; J C Wilber; P Johnson; L Tobler; C S Evans
Journal:  Transfusion       Date:  1992-06       Impact factor: 3.157

9.  Detection of hepatitis C virus RNA by a combined reverse transcription-polymerase chain reaction assay.

Authors:  K K Young; R M Resnick; T W Myers
Journal:  J Clin Microbiol       Date:  1993-04       Impact factor: 5.948

10.  Detection of hepatitis C viral sequences in blood donations by "nested" polymerase chain reaction and prediction of infectivity.

Authors:  J A Garson; R S Tedder; M Briggs; P Tuke; J A Glazebrook; A Trute; D Parker; J A Barbara; M Contreras; S Aloysius
Journal:  Lancet       Date:  1990-06-16       Impact factor: 79.321
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