Literature DB >> 8735122

Detection of hepatitis E virus RNA in stools and serum by reverse transcription-PCR.

S Turkoglu1, Y Lazizi, H Meng, A Kordosi, P Dubreuil, B Crescenzo, S Benjelloun, P Nordmann, J Pillot.   

Abstract

Stools and sera collected during an experimental hepatitis E virus (HEV) infection in monkeys and collected from humans with acute HEV infections during epidemic and sporadic cases were analyzed by reverse transcription-PCR. Two methods for RNA purification were compared. Proteinase K digestion and phenolchloroform extraction were more efficient than guanidinium isothiocyanate extraction in improving the sensitivity and specificity for the detection of HEV genomes.

Entities:  

Mesh：

Substances：

Year:  1996        PMID： 8735122      PMCID： PMC229066          DOI： 10.1128/jcm.34.6.1568-1571.1996

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  7 in total

1.  Isolation of a cDNA from the virus responsible for enterically transmitted non-A, non-B hepatitis.

Authors:  G R Reyes; M A Purdy; J P Kim; K C Luk; L M Young; K E Fry; D W Bradley
Journal:  Science       Date:  1990-03-16       Impact factor: 47.728

2.  Improved amplification of cytomegalovirus DNA from urine after purification of DNA with glass beads.

Authors:  G J Buffone; G J Demmler; C M Schimbor; J Greer
Journal:  Clin Chem       Date:  1991-11       Impact factor: 8.327

Review 3.  Report of a workshop: waterborne non-A, non-B hepatitis.

Authors:  I D Gust; R H Purcell
Journal:  J Infect Dis       Date:  1987-10       Impact factor: 5.226

4.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

Authors:  P Chomczynski; N Sacchi
Journal:  Anal Biochem       Date:  1987-04       Impact factor: 3.365

5.  Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactions.

Authors:  J Wilde; J Eiden; R Yolken
Journal:  J Clin Microbiol       Date:  1990-06       Impact factor: 5.948

6.  Experimental hepatitis E: pathogenesis in cynomolgus macaques (Macaca fascicularis).

Authors:  C F Longer; S L Denny; J D Caudill; T A Miele; L V Asher; K S Myint; C C Huang; W F Engler; J W LeDuc; L N Binn
Journal:  J Infect Dis       Date:  1993-09       Impact factor: 5.226

7.  Variation in course of hepatitis E in experimentally infected cynomolgus monkeys.

Authors:  S A Tsarev; S U Emerson; T S Tsareva; P O Yarbough; M Lewis; S Govindarajan; G R Reyes; M Shapiro; R H Purcell
Journal:  J Infect Dis       Date:  1993-06       Impact factor: 5.226
  7 in total
  2 in total

1.  [Incidence of sporadic hepatitis E in Ivory Coast based on still problematic serology].

Authors:  M Rioche; P Dubreuil; A Kouassi-Samgare; V Akran; P Nordmann; J Pillot
Journal:  Bull World Health Organ       Date:  1997       Impact factor: 9.408

2.  Prolonged fecal shedding of hepatitis E virus (HEV) during sporadic acute hepatitis E: evaluation of infectivity of HEV in fecal specimens in a cell culture system.

Authors:  Masaharu Takahashi; Toshinori Tanaka; Masahiro Azuma; Eiji Kusano; Tatsuya Aikawa; Takao Shibayama; Yasuyuki Yazaki; Hitoshi Mizuo; Jun Inoue; Hiroaki Okamoto
Journal:  J Clin Microbiol       Date:  2007-08-29       Impact factor: 5.948
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.