BACKGROUND: A viral etiology has been suspected in papillomatosis of the external auditory canal (PEAC), but virus particles have not been detected so far, although they are easily demonstrable in skin warts. The purpose of the study was to solve this discrepancy by the use of polymerase chain reaction (PCR). MATERIALS AND METHODS: Specimens from the external auditory canal of 14 patients with PEAC, but no human papilloma virus infection of the genital areas, were examined histologically by light and electron microscopy, as well as by PCR to detect viral DNA. RESULTS: Histologically, papillomatosis was present in all specimens. Vacuolated cells were found in the upper part of the stratum malpighii in five cases. On electron microscopy, the numbers of perichromatin and interchromatin granules were increased, but no viral granules were observed. In all specimens, DNA of HPV 6 was detected using PCR, but there was no evidence of DNA of other HPV. CONCLUSIONS: Papilloma of the external auditory canal is produced by infection with HPV 6.
BACKGROUND: A viral etiology has been suspected in papillomatosis of the external auditory canal (PEAC), but virus particles have not been detected so far, although they are easily demonstrable in skin warts. The purpose of the study was to solve this discrepancy by the use of polymerase chain reaction (PCR). MATERIALS AND METHODS: Specimens from the external auditory canal of 14 patients with PEAC, but no human papilloma virus infection of the genital areas, were examined histologically by light and electron microscopy, as well as by PCR to detect viral DNA. RESULTS: Histologically, papillomatosis was present in all specimens. Vacuolated cells were found in the upper part of the stratum malpighii in five cases. On electron microscopy, the numbers of perichromatin and interchromatin granules were increased, but no viral granules were observed. In all specimens, DNA of HPV 6 was detected using PCR, but there was no evidence of DNA of other HPV. CONCLUSIONS:Papilloma of the external auditory canal is produced by infection with HPV 6.
Authors: Jun Hee Lee; Jin Sik Burm; Won Yong Yang; Sang Yoon Kang; Sung Wan Byun Journal: Clin Exp Otorhinolaryngol Date: 2015-08-13 Impact factor: 3.372
Authors: Samir Cahali; Flávia B da Silva; Márcia C Machado; Danielle A da Silva; Olga M R Reforeme; Michel B Cahali Journal: Braz J Otorhinolaryngol Date: 2005-12-14