Literature DB >> 8727932

New drug susceptibility test for Mycobacterium tuberculosis using the hybridization protection assay.

J Miyamoto1, H Koga, S Kohno, T Tashiro, K Hara.   

Abstract

We developed a novel method for early detection of drug-resistant strains of Mycobacterium tuberculosis by using the hybridization protection assay (HPA). The number of viable bacteria during the incubation period correlated well with the number of relative light units measured by the HPA. In addition, the relative light unit values of susceptible strains on the first, third and fifth days of incubation were significantly different from those of resistant strains for both isoniazid and rifampin. Our results suggest that after isolation of the organism from clinical specimens, drug-resistant strains of M. tuberculosis are accurately detected by the HPA even after 1 day of incubation with the drug.

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Year:  1996        PMID： 8727932      PMCID： PMC229011          DOI： 10.1128/jcm.34.5.1323-1326.1996

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  22 in total

1.  Anti-Mycobacterium avium activity of quinolones: in vitro activities.

Authors:  G Klopman; S Wang; M R Jacobs; S Bajaksouzian; K Edmonds; J J Ellner
Journal:  Antimicrob Agents Chemother       Date:  1993-09       Impact factor: 5.191

2.  Detection of Mycobacterium tuberculosis in clinical specimens by polymerase chain reaction and Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test.

Authors:  C Abe; K Hirano; M Wada; Y Kazumi; M Takahashi; Y Fukasawa; T Yoshimura; C Miyagi; S Goto
Journal:  J Clin Microbiol       Date:  1993-12       Impact factor: 5.948

3.  Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by amplification of rRNA.

Authors:  V Jonas; M J Alden; J I Curry; K Kamisango; C A Knott; R Lankford; J M Wolfe; D F Moore
Journal:  J Clin Microbiol       Date:  1993-09       Impact factor: 5.948

4.  Direct detection of Mycobacterium tuberculosis complex in respiratory specimens by a target-amplified test system.

Authors:  G E Pfyffer; P Kissling; R Wirth; R Weber
Journal:  J Clin Microbiol       Date:  1994-04       Impact factor: 5.948

5.  Evaluation of the BACTEC radiometric method for recovery of mycobacteria and drug susceptibility testing of Mycobacterium tuberculosis from acid-fast smear-positive specimens.

Authors:  G D Roberts; N L Goodman; L Heifets; H W Larsh; T H Lindner; J K McClatchy; M R McGinnis; S H Siddiqi; P Wright
Journal:  J Clin Microbiol       Date:  1983-09       Impact factor: 5.948

6.  inhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis.

Authors:  A Banerjee; E Dubnau; A Quemard; V Balasubramanian; K S Um; T Wilson; D Collins; G de Lisle; W R Jacobs
Journal:  Science       Date:  1994-01-14       Impact factor: 47.728

7.  In vitro susceptibilities of rapidly growing mycobacteria to newer antimicrobial agents.

Authors:  N Khardori; H Nguyen; B Rosenbaum; K Rolston; G P Bodey
Journal:  Antimicrob Agents Chemother       Date:  1994-01       Impact factor: 5.191

8.  Rapid drug-susceptibility testing of Mycobacterium tuberculosis.

Authors:  D E Snider; R C Good; J O Kilburn; L F Laskowski; R H Lusk; J J Marr; Z Reggiardo; G Middlebrook
Journal:  Am Rev Respir Dis       Date:  1981-04

9.  Interlaboratory drug susceptibility testing of Mycobacterium tuberculosis by a radiometric procedure and two conventional methods.

Authors:  S H Siddiqi; J E Hawkins; A Laszlo
Journal:  J Clin Microbiol       Date:  1985-12       Impact factor: 5.948

10.  Assessment of mycobacterial viability by RNA amplification.

Authors:  G M van der Vliet; P Schepers; R A Schukkink; B van Gemen; P R Klatser
Journal:  Antimicrob Agents Chemother       Date:  1994-09       Impact factor: 5.191
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  4 in total

1.  Rapid method for testing susceptibility of Mycobacterium tuberculosis by using DNA probes.

Authors:  N Martin-Casabona; D Xairó Mimó; T González; J Rossello; L Arcalis
Journal:  J Clin Microbiol       Date:  1997-10       Impact factor: 5.948

2.  Molecular bacterial load assay, a culture-free biomarker for rapid and accurate quantification of sputum Mycobacterium tuberculosis bacillary load during treatment.

Authors:  Isobella Honeyborne; Timothy D McHugh; Patrick P J Phillips; Selina Bannoo; Anna Bateson; Nora Carroll; Felicity M Perrin; Katharina Ronacher; Laura Wright; Paul D van Helden; Gerhard Walzl; Stephen H Gillespie
Journal:  J Clin Microbiol       Date:  2011-09-07       Impact factor: 5.948

3.  Quantitative analysis of mRNA as a marker for viability of Mycobacterium tuberculosis.

Authors:  T J Hellyer; L E DesJardin; G L Hehman; M D Cave; K D Eisenach
Journal:  J Clin Microbiol       Date:  1999-02       Impact factor: 5.948

4.  Early detection of mycobacteria using a novel hydrogel culture method.

Authors:  Mi Hee Jang; Shine Young Kim; Chang-Ki Kim; Sang-Hyun Hwang; Byung Kyu Park; Sung Soo Kim; Eun Yup Lee; Chulhun L Chang
Journal:  Ann Lab Med       Date:  2013-12-06       Impact factor: 3.464
  4 in total

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